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4-Fluoro-7-nitrobenzofurazan CAS:29270-56-2
4-Fluoro-7-nitrobenzofurazan ni imvange y’ibinyabutabire ifite formula ya molekile C6H2FN3O3. Ni ikintu cy’umuhondo gikozwe muri kristale gikoreshwa cyane cyane nk’ikintu kigira ingaruka ku mikorere itandukanye ya shimi. 4-Fluoro-7-nitrobenzofurazan izwiho ubushobozi bwo gukorana n’amine z’ibanze, igakora ibintu bivamo fluorescent bishobora kugira akamaro mu isesengura nko gushyira ibirango bya poroteyine no gusesengura aside amine. Ikoreshwa kandi mu bushakashatsi bujyanye na kinetics ya enzyme no kumenya urukurikirane rw’aside nucleic.
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5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium umunyu CAS: 18656-96-7
5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide Umunyu wa cyclohexylammonium, uzwi kandi ku izina rya X-glucuronide, ni ikintu gikoreshwa mu bushakashatsi bwa biology ya molekile na enzymology. Ni substrate ya chromogenic ikunze gukoreshwa mu kumenya imikorere ya enzyme beta-D-glucuronidase.
Iyi mvange igizwe n'itsinda rya indole rihujwe n'igice cya aside glucuronic binyuze mu mugozi wa glycosidic. Bromo na chloro bisimbura ibinyabutabire bitanga ituze kandi byongera ubushobozi bwo gushonga kw'iyi mvange.
Iyo ishyizwe mu gipimo kirimo beta-D-glucuronidase, enzyme igabanya umugozi wa glycosidic, irekura igice cya indole. Iyi reaction ituma habaho ihinduka ry'ubururu cyangwa ibara, bigatuma byoroshye kubona imikorere ya enzyme mu buryo bw'amaso cyangwa spectrophotometric.
Bitewe n'uburyo ikoresha beta-D-glucuronidase mu buryo bwihariye, X-glucuronide ikoreshwa cyane mu buhanga bwa molekile nka bagiteri n'impinduka z'umusemburo, isuzuma rya gene, no gusiga irangi rya histochemical. Itanga uburyo bworoshye kandi bworoshye bwo kumenya niba beta-D-glucuronidase ihari cyangwa igaragara, kandi ikunze gukoreshwa hamwe n'ibindi bipimo bya gene cyangwa biochemical.
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8-Anilino-1-aside naphthalenesulfonike CAS: 82-76-8
8-Anilino-1-naphthalenesulfonic aside, izwi kandi nka ANS, ni irangi ry’urumuri rikunze gukoreshwa mu bushakashatsi bwa biochemical na biophysical. Riri mu bwoko bw’ibinyabutabire by’umwimerere bizwi nka aside sulfonic zishingiye kuri aniline. ANS izwiho ubushobozi bwo kwihuza n’uturere duto duto cyangwa ahantu hagaragaramo hydrophobic muri poroteyine n’izindi biomolecules.
ANS irahinduka iyo ifatanye n'uturere duto duto duto, bigatuma iba igikoresho cy'ingirakamaro cyo kwiga impinduka mu miterere ya poroteyine, imikoranire ya poroteyine na poroteyine, n'uburyo poroteyine zigenda zipfunyika/zigasohoka. Guhuza ANS n'uturere duto duto duto bituma habaho impinduka y'ubururu mu buryo bw'ibyuka bihumanya, bishobora gukurikiranwa hakoreshejwe spectroscopy ya fluorescence.
ANS ikunze gukoreshwa mu buryo butandukanye bw'ubushakashatsi, harimo fluorescence polarization, fluorescence resonance energy transfer (FRET), no gusuzuma ubushyuhe. Yakoreshejwe mu kwiga imiterere n'ubudahangarwa bwa poroteyine, imikoranire ya poroteyine na ligand, hamwe n'uburyo poroteyine ziteranywa.
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CHLOROPHENOLRED-BETA-D-GALACTOPIRANOSIDE, UMUNYU WA SODIYUMU CAS:99792-50-4
CHLOROPHENOLRED-BETA-D-GALACTOPIRANOSIDE, UMUNYU WA SODIYUMU ni ikinyabutabire gikunze gukoreshwa mu bushakashatsi bwa laboratwari kugira ngo hamenyekane niba hari imisemburo ishobora gusenya lactose, nka beta-galactosidase. Ni ifu idafite ibara cyangwa umuhondo werurutse ihinduka umutuku iyo hari izi misemburo, bigatuma iba ikimenyetso cy'ingirakamaro cy'imikorere yazo. Ubwoko bw'umunyu wa sodiyumu w'iki kinyabutabire butuma gishonga neza mu bisubizo by'amazi, bigatuma byoroha gutegura no gukoresha mu bipimo bitandukanye bya enzyme.
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Tert-Butylamine CAS 75-64-9 – Ubwiza bwizewe ku bikoresho bigezweho
Tert-Butylamine (CAS 75-64-9) iratandukanye kubera ubuziranenge bwayo bwo hejuru, imikorere yayo ihoraho, hamwe no kudahinduka kw'imiti. Iyi amine ifite uburyo bwinshi bwo kuyihindura irazwi cyane kubera ubushobozi bwayo bwo gushonga no guhuza neza n'izindi nganda zitandukanye. Ibyiza by'ingenzi birimo kuba ifite ubushobozi bwo kuba inkingi y'ubwubatsi mu gukora imvange y'ibinyabuzima, kuba urwego rwo hagati mu gukora imiti, no kwihutisha gutunganya kabutura. Tert-Butylamine kandi ibona akamaro mu binyabutabire by'ubuhinzi no mu kwirinda ingese mu mavuta. Guhitamo ibicuruzwa byacu bivuze gukorana n'inzobere zishyira imbere kugenzura ubuziranenge no gutanga ibisubizo by'abakiriya, zikerekana ko ubona serivisi yizewe kandi ku gihe hamwe n'ubufasha bwa tekiniki. Urashaka kongera imikorere mu mushinga wawe utaha? Twandikire noneho tuganire ku byo ukeneye!
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Neocuproine CAS 484-11-7 – Uburyo bwo gukora ubushakashatsi buhanitse
Neocuproine (CAS 484-11-7) igaragara nk'ikintu cyihariye kandi gifite isuku nyinshi kandi kizwiho guhitamo neza iyoni z'umuringa (I). Ihamye yayo ikomeye mu bya shimi n'imiterere yayo ya kristale ituma ikundwa cyane haba muri laboratwari z'amashuri n'inganda. Ibyiza by'ingenzi birimo imikorere yayo yizewe mu bushakashatsi bwa spectroscopic, ubushakashatsi ku binyabuzima, no gusesengura, ndetse no guhuza n'uburyo butandukanye bwo gusesengura no gukora ibintu. Ingufu za Neocuproine zemejwe ko zorohereza iterambere ry'uburyo bwo gushakisha bwihuse n'uburyo bushya bwo gushakisha mu bushakashatsi bwa shimi na biochemical. Guhitamo twe bituma ubona ubuziranenge bupimwe neza, amasoko agaragara, n'ubufasha bw'inzobere mu bya tekiniki bujyanye n'ibyo ukeneye byihariye. Niba wifuza kumenya uburyo Neocuproine ishobora kunoza umushinga wawe, ntutindiganye kutwandikira—iterambere ryawe ritaha ry'ubushakashatsi rishobora gutangirira hano. Nyandikira uyu munsi kugira ngo tuganire ku byo ukeneye cyangwa usabe ikiguzi!
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Tapso Sodium CAS: 105140-25-8 Igiciro cy'umukozi
aside 3-[N-Tris(hydroxymethyl)methylamino]-2-hydroxypropanesulfonic,Ni njyesa zwitterionic buffering agent mu buryo bw'umunyu wa sodium. Ikunze gukoreshwa mu bushakashatsi bwa biochemical na molekile kugira ngo pH ikomeze kuba ihamye mu bisubizo.It Irashongesha cyane mu mazi kandi ifite pKa hafi ya pH y’umubiri, bigatuma ikoreshwa mu buryo butandukanye nko gutunganya poroteyine, gusuzuma enzymes, gusesengura uturemangingo, no gukoresha electrophoresis. Imiterere yayo yihariye ya shimi n’uburyo ihura na biology bituma iba ingenzi mu mabwiriza atandukanye ya laboratwari.
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4-Nitrophenyl phosphate di(tris) umunyu CAS:68189-42-4
Umunyu wa 4-Nitrophenyl phosphate di(tris) ni ikinyabutabire gikoreshwa mu bushakashatsi no gusuzuma ibinyabutabire. Ukunze gukoreshwa nk'ishingiro ry'ibizamini bya alkaline phosphatase. Uyu munyu, iyo ukozwe hidrolised na alkaline phosphatase, ukora ikintu gifite ibara ry'umuhondo gishobora gupimwa byoroshye hifashishijwe spectrophotometric. Iki gikorwa gikoreshwa cyane mu bikorwa bitandukanye, harimo ibizamini bya enzyme-linked immunosorbent assays (ELISAs) no kumenya ibikorwa bya phosphatase. Ubukana bw'ibara ry'umuhondo bungana neza n'ibikorwa bya enzyme, bigatuma haboneka neza urugero rwa alkaline phosphatase mu bipimo by'ibinyabuzima. Umunyu wa 4-Nitrophenyl phosphate di(tris) urahamye, ushonga mu mazi, kandi urushaho kugira ingaruka nziza, bigatuma uba amahitamo akunzwe yo gupima enzymes mu bushakashatsi bw'ibinyabuzima n'ubw'ubuvuzi.
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Nitrotetrazolium blue chloride CAS:298-83-9
Nitrotetrazolium blue chloride, izwi kandi nka NBT cyangwa NBT-Cl, ni ikinyabutabire gikunze gukoreshwa mu isuzuma ry’ibinyabuzima n’ibinyabutabire. Ni ifu y’umuhondo werurutse ishongeshwa mu mazi.
NBT ikunze gukoreshwa nk'ikimenyetso cyo kumenya niba hari superoxide radicals cyangwa enzymes zibikora. Ihinduka ibara iyo igabanutse na superoxide radicals, ikava ku muhondo ikajya ku bururu bwijimye bwa formazan. Iyi reaction ikunze gukoreshwa mu bushakashatsi bwo kwiga stress ya oxidative, guhumeka kw'uturemangingo, n'imikorere ya enzymes.
NBT ikunze gukoreshwa mu buryo butandukanye, nko kugabanya nitroblue tetrazolium, kugira ngo igaragaze ikorwa rya superoxide mu turemangingo no mu ngingo. Yanakoreshejwe muri immunohistochemistry na histochemistry kugira ngo hamenyekane kandi hamenyekane izindi enzymes n'ibice bigize uturemangingo.
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NSP-AS CAS:211106-69-3 Igiciro cy'umukozi
Umunyu w'imbere wa 3-[9-((3-(carboxypropyl)[4-Methxylphenyl]sulfonyl)aMine)carboxyl]-10-acridiniuMyl)-1-propanesulfonate ni molekile igizwe n'igice cya acridinium gifatanye n'umunyu w'imbere wa propanesulfonate. Irimo itsinda rya carboxypropyl, itsinda rya 4-Methxylphenylsulfonyl, n'itsinda rya amine. Iki gice gishobora kuba cyarakorewe gukoreshwa mu buryo bwihariye nko mu butabire, mu by'imiti, cyangwa mu bushakashatsi.
Byongeye kandi, NAC yagaragaje icyizere mu gushyigikira ubuzima bw'umwijima ifasha mu gukuraho ibintu by'uburozi, nka acetaminophen, umuti usanzwe ugabanya ububabare. Ishobora kandi kugira ingaruka zo kurinda umwijima kwangirika guterwa no kunywa inzoga.
Uretse kuba ifite ubushobozi bwo kurwanya indwara ziterwa n’ubudahangarwa bw’umubiri (antioxidant) n’ubufasha mu guhumeka, NAC yanasuzumwe ku nyungu zayo mu buzima bwo mu mutwe. Ubushakashatsi bumwe bwerekana ko ishobora kugira ingaruka nziza ku ndwara zo mu mutwe, nko kwiheba no kudakora ibintu bikunze kugorama (OCD).
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L-Leucine p-nitroanilide hydrochloride CAS: 16010-98-3
L-Leucine p-nitroanilide hydrochloride ni ikinyabutabire kiri mu itsinda ry'ibikomoka kuri p-nitroanilide. Ni ifu y'umweru kugeza ku umweru ikoreshwa cyane mu bushakashatsi bwa biochemical no mu isuzuma rya enzymes.
L-Leucine p-nitroanilide hydrochloride ikunze gukoreshwa nk'igice cyo gupima imikorere ya enzymes nka poroteyine na peptidase. Iyo izi enzymes zivanzemo L-Leucine p-nitroanilide hydrochloride, isohora p-nitroaniline, ishobora kugaragara hifashishijwe spectrophotometric. Igipimo cy'impinduka z'amabara gihuye neza n'imikorere ya enzymes, bigatuma umubare w'imikorere ya enzymes mu bipimo by'ibinyabuzima upimwa.
Iki kintu kirashongeshwa mu mazi kandi gishobora gushongeshwa byoroshye mu bisubizo bikwiye byo gukoreshwa mu gusuzuma enzymes. Ni ngombwa kubika L-Leucine p-nitroanilide hydrochloride ahantu hakonje kandi humutse kugira ngo gikomeze kuba cyiza.
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Guanidine Thiocyanate Sulfate CAS: 593-84-0
Sulfate ya Guanidine thiocyanate ni imvange y’ibinyabutabire ikomoka kuri iyoni za guanidine, thiocyanate, na sulfate. Ikunze gukoreshwa mu bushakashatsi bwa biology ya molekile na biochimie nk'ikintu gitera chaotropic.
Guanidine thiocyanate sulfate ikunze gukoreshwa muri gahunda zo gutandukanya ADN na RNA kugira ngo ihagarike uturemangingo tw’uturemangingo na poroteyine zidafite ubuziranenge, bigatuma aside nucleic zikurwamo neza. Ifasha mu gushonga kwa ADN na RNA, bigatuma bikurwamo kandi bigatunganywa mu buryo butandukanye.
Bitewe n’imiterere yayo ya chaotropic, guanidine thiocyanate sulfate ifasha mu gusenya hydrogen bond n’ibindi bikorwa bitari covalent, yorohereza gutandukanya aside nucleic na poroteyine, lipide, n’ibindi bice by’uturemangingo. Ifasha kandi mu kubuza imikorere ya RNase, ikerekana ubuziranenge n’ubuziranenge bwa aside nucleic zakuwemo..
