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Ninhydrine hydrate CAS: 485-47-2 Igiciro cy'umukozi
Ninhydrine hydrate ni imvange y'ibinyabutabire ikunze gukoreshwa mu isesengura ry'ubutasi n'ubutabire bw'ibinyabuzima. Ni ifu y'umuhondo woroshye idafite ibara cyangwa ishongesha mu mazi no mu binyabutabire by'ibinyabuzima.
Ninhydrine hydrate izwiho ubushobozi bwo gukorana na aside amine na amine z’ibanze, bigatuma habaho ibara ry’umuhengeri n’ubururu. Iyi reaction ikunze gukoreshwa mu isesengura ry’ibikumwe bya forensic kugira ngo harebwe kandi hongererwe uburyo ibikumwe byahishwe bigaragara ku buso bufite imyenge. Umuti wa ninhydrine ukunze gusukwa ku buso, kandi ibikumwe bikura uko igihe kigenda gihita uko ninhydrine ikorana na aside amine ziri mu bisigazwa by’ibikumwe.
Muri chimie y’ibinyabuzima, ninhydrine hydrate ikoreshwa nk'ikintu kivura aside amine, peptides na poroteyine. Ishobora gukoreshwa mu kumenya niba aside amine ziri mu gipimo no kubara ingano ya aside amine. Uburyo ninhydrine ikora butuma habaho ikintu gifite ibara, gishobora gupimwa hifashishijwe spectrophotometric kugira ngo hamenyekane ingano y’umusesenguzi.
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D-Luciferine CAS: 2591-17-5 Igiciro cy'umukozi
D-Luciferine ni ikintu gito gitanga urumuri kiboneka cyane cyane mu binyabuzima bitanga urumuri nk'udusimba tw'urumuri, bagiteri, n'ibinyabuzima byo mu mazi. Ni cyo kintu cy'ingenzi mu gukora luciferase reaction, inzira ya biochemical itanga urumuri.
D-Luciferine ikunze gukoreshwa nk'ikintu cy'ingenzi mu isuzuma rya bioluminescence no mu buhanga bwo gufata amashusho, aho uburyo ikora hamwe na luciferase butanga urumuri rushobora kuboneka no gupimwa. Ibi bituma iba igikoresho cy'ingirakamaro mu nzego zitandukanye z'ubushakashatsi, harimo ibinyabuzima by'ibinyabuzima, kuvumbura imiti, no gufata amashusho mu buryo bw'ubuvuzi.
Bitewe n'imiterere yayo yihariye y'amaso, D-Luciferin yakoreshejwe cyane mu bushakashatsi bujyanye no kugaragaza ingirabuzimafatizo, imikoranire ya poroteyine na poroteyine, no gutanga ibimenyetso ku turemangingo. Itanga uburyo budakoresha ikoranabuhanga kandi bwihuse bwo gusuzuma imikorere y'ibinyabuzima mu gihe nyacyo.
Byongeye kandi, hakozwe ibintu biva kuri D-Luciferin na analogues, bituma habaho impinduka zitandukanye n'imikoreshereze. Ibi bintu biva kuri D-Luciferin bihindura imiterere y'urumuri, bigatuma habaho gutuza no kwinjira neza mu mubiri no mu mubiri.
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aside p-hydroxybenzoic, monosodium CAS: 114-63-6
Aside p-Hydroxybenzoic, monosodium (izwi kandi nka sodium 4-hydroxybenzoate) ni imvange y’ibinyabutabire ifite formula ya molekile C7H5NaO3. Ni umunyu wa sodium ukomoka kuri aside p-hydroxybenzoic, ikaba ari imvange karemano iboneka mu bimera nk'imbuto n'imboga.
Aside p-Hydroxybenzoic, monosodium ikoreshwa mu nganda zitandukanye kubera ubushobozi bwayo bwo kurwanya indwara. Ikunze gukoreshwa nk'ikintu kirinda kwangirika mu biribwa, ibinyobwa, amavuta yo kwisiga, n'ibikoresho byo kwiyitaho kugira ngo ikumire ikwirakwira rya bagiteri, ibihumyo n'umusemburo. Ifasha kongera igihe cyo kumara ibi bicuruzwa mu kwirinda kwangirika no kubungabunga ubuziranenge bwabyo.
Mu mikorere y'imiti, aside p-Hydroxybenzoic, monosodium ikoreshwa nk'umusemburo mu miti inyobwa no mu miti ikoreshwa mu mubiri. Imiterere yayo irwanya udukoko ifasha mu kubungabunga umutekano no kutandura kw'ibi bicuruzwa, bigatuma birushaho kugira umutekano n'ubushobozi.
Byongeye kandi, aside p-Hydroxybenzoic, monosodium ifite ubushobozi bwo kurwanya okisidasiyo, ituma igira akamaro mu gukumira kwangirika kwa okisidasiyo mu bicuruzwa bitandukanye. Ishobora gufasha mu kurinda kwangirika kw'ibintu no kongera igihe cyose cy'ibicuruzwa..
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4-Nitrophenyl phosphate disodium salt hexahydrate CAS: 4264-83-9
4-Nitrophenylphosphate disodium salt hexahydrate ni ikinyabutabire gikunze gukoreshwa nk'ishingiro ryo kumenya imikorere ya enzymes za fosfati. Kigaragara nk'ifu y'umweru kuva ku ifu y'umweru kugeza ku ifu y'umweru kandi gishongesha cyane mu mazi. Iyo enzymes za fosfati zikoreshejwe, zihura n'ingaruka zituma habaho ibara ry'umuhondo, rishobora gupimwa hifashishijwe spectrophotometric. Iki kinyabutabire gikoreshwa mu gusuzuma no gupima imikorere ya fosfati mu bipimo..
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Methylphenazinium methosulfate CAS:299-11-6
Methylphenazinium methosulfate (MPMS) ni ikintu gikora muri redox gikunze gukoreshwa nk'igikoresho gitwara electron mu bushakashatsi butandukanye bwa biochemical na biophysical. Ni umunyu ugizwe na methylphenazinium cation (ikintu kigizwe n'umwuka wa heterocyclic) na methosulfate anion.
MPMS ikunze gukoreshwa nk'uburyo bwo gusimbura electron zisanzwe, nka ferricyanide cyangwa phenazine ethosulfate, bitewe nuko ihamye kandi ishonga cyane mu mazi. Ifite ubushobozi bwiza bwo gukosora imiterere y'amaraso, butuma yakira kandi igatwara electron mu gihe cy'ibikorwa bya enzyme.
Imwe mu mikoreshereze y'ingenzi ya MPMS ni mu bipimo bikubiyemo gupima uburyo electron ihererekanya cyangwa imikorere ya enzyme. Ikunze gukoreshwa hamwe na sisitemu ya enzyme kugira ngo ikurikirane uburyo electron zihererekanya hagati y'ibice bitandukanye. Kugabanuka kwa MPMS bishobora kugaragara hifashishijwe spectrophotometric, aho uburyo ifata buhinduka bitewe n'uburyo electron ihererekanya.
MPMS ikoreshwa kandi mu bushakashatsi bujyanye no guhumeka kwa mitochondrial na phosphorylation ya oxidative. Ishobora gukora nk'igikoresho cy'ubukorano cyakira electron, bigatuma abashakashatsi bakora ubushakashatsi ku mikorere n'uburyo izi nzira zikoreshwa mu buryo butandukanye bw'ibinyabuzima.
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4-Nitrophenyl-alpha-L-fucopyranoside CAS: 10231-84-2
4-Nitrophenyl-alpha-L-fucopyranoside ni ikinyabutabire kiri mu muryango wa glycosides. Kigizwe n'umusemburo w'isukari ya fucose ufatanye n'itsinda rya 4-nitrophenyl. Iki kinyabutabire gikunze gukoreshwa nk'igice cy'ingenzi mu isuzuma rya enzymatic kugira ngo higwe imikorere ya fucosidases, ari zo enzymes zigira uruhare mu gusenyuka kwa molekile zirimo fucose. Iyo enzyme ya fucosidase ikoreshejwe, 4-nitrophenyl-alpha-L-fucopyranoside iracikamo ibice, bigatuma 4-nitrophenol irekurwa, ishobora gupimwa hakoreshejwe spectrophotometry. Iki gice cy'ingenzi cyane mu bushakashatsi bujyanye n'imikorere ya enzymatic, umwihariko wa substrate, gusuzuma inzitizi, n'uburyo enzymes za fucosidase zikora.
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N-Ethylmaleimide CAS:128-53-0 Igiciro cy'umukozi
N-Ethylmaleimide (NEM) ni ikintu gito cy’ibinyabuzima gikunze gukoreshwa mu bushakashatsi bwa biochimie na molekile. Ikora nk'ikintu cyihariye kibuza amatsinda ya poroteyine sulfhydryl (thiol) mu guhindura no guhagarika imikorere yayo mu buryo budasubirwaho. NEM ikora cyane ku matsinda ya sulfhydryl, nk'ayaboneka muri aside amine cysteine, kandi ishobora gukorana n'amatsinda ya sulfhydryl yigenga ndetse n'ayarimo poroteyine. Ibi bituma NEM iba igikoresho cy'ingirakamaro mu kwiga imikorere ya poroteyine, imikoranire ya poroteyine na poroteyine, n'imikorere ya enzyme. Imiterere yayo yo kuyibuza yakoreshejwe mu buryo butandukanye, harimo proteomics, enzymology, biology structural, no kuvumbura imiti.
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5-Aside Sulfosalicylic dihydrate CAS: 5965-83-3
5-Aside Sulfolicylic dihydrate ni igikoresho cy’ibinyabutabire gikunze gukoreshwa muri laboratwari za biochemical na farumasi. Ni ikintu cy’umweru gikozwe muri kristu gishongesha cyane mu mazi. 5-Aside Sulfolicylic dihydrate ni ikintu gikomoka kuri aside salicylic kandi gifite formula ya molekile ya C7H6O6S. Ikunze gukoreshwa nk'ikintu gifasha mu kuvura poroteyine kandi ishobora gukoreshwa mu gupima poroteyine mu bipimo by’ibinyabuzima, nk'inkari na serum. Byongeye kandi, ishobora gukoreshwa mu kugena ibintu bitandukanye, harimo aside nucleic, enzymes, hormones, n'imiti. Imiterere ya dihydrate y'icyo kintu igaragaza ko kirimo molekile ebyiri z'amazi kuri molekile ya aside.
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Iodide ya potasiyumu CAS: 7681-11-0
Iodide ya potasiyumu (KI) ni ikintu kidakomoka ku bimera kigizwe na potassium cations (K+) na anions ya iyode (I-). Ni ikintu cy'umweru, gifite kristali gishonga cyane mu mazi. Iodide ya potasiyumu ifite akamaro n'ikoreshwa ritandukanye bitewe n'imiterere yayo.
Imwe mu mikoreshereze y'ingenzi ya iyode potasiyumu ni mu buvuzi. Ikunze gukoreshwa nk'inyongera mu kuvura no gukumira indwara ziterwa no kubura iyode, nka goiter, imisemburo idahwitse ya tiroyide, hamwe n'ubwoko bumwe na bumwe bwa kanseri ya tiroyide. Ishobora kandi gukoreshwa nk'ubuvuzi bwihutirwa ku bijyanye n'imirasire, kuko ifasha gukumira iyode iterwa na tiroyide.
Iyode ya potasiyumu ikoreshwa kandi muri laboratwari. Ikoreshwa nk'ikintu kivura ubukana mu gusesengura shimi kugira ngo hamenyekane niba hari ibintu bimwe na bimwe, nka lisansi na mercure, binyuze mu gukora utubumbe tw'umuhondo tudashonga. Byongeye kandi, ishobora gukoreshwa nk'isoko ya iyode mu buryo butandukanye bwo gukora imiti.
Mu rwego rwo guteka, iyode ya potasiyumu rimwe na rimwe yongerwa mu munyu wo ku meza (umunyu wa iyode) mu rwego rwo kwirinda ibura rya iyode mu baturage. Umunyu wa iyode ukoreshwa n'abantu benshi ku isi nk'isoko y'ibiribwa bya iyode.
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8-Anilino-1-naphthalenesulfonic aside ammonium umunyu CAS: 28836-03-5
8-Anilino-1-aside naphthalenesulfonike Umunyu wa ammonium ni ikinyabutabire gikunze gukoreshwa nk'irangi ritanga urumuri. Gifite imiterere igizwe n'itsinda rya aniline rifatanye na molekyuli ya aside naphthalenesulfonike hamwe n'umunyu wa ammonium. Iki kinyabutabire kirashongeshwa mu mazi kandi gitanga imbaraga zo kwinjiza no gusohora urumuri mu buryo bugaragara.
Bitewe n'ubushobozi bwayo bwo gushyuha, umunyu wa aside ammonium ya 8-Anilino-1-naphthalenesulfonic ukunze gukoreshwa nk'icyitegererezo cyangwa ikimenyetso mu bushakashatsi butandukanye bwa biochemical na biophysical. Urashobora gukoreshwa mu kumenya impinduka muri pH, impinduka mu miterere ya poroteyine, imikoranire ya poroteyine na ligand, ndetse no kuba hari ions zimwe na zimwe.
Iyo ishyushye n'urumuri rukwiye, iyi mvange itanga urumuri rw'ubururu bwerurutse, bigatuma byoroha kuyibona no kuyipima. Imiterere yayo y'urumuri ituma iba igikoresho cy'ingirakamaro mu nzego z'ubushakashatsi nka biology ya molekile, biochimie, na biology ya selile.
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Sodium dodecyl sulfate CAS: 151-21-3
Sodium dodecyl sulfate (SDS) ni surfactant ikoreshwa cyane mu nganda zitandukanye no mu bushakashatsi. Ni ikintu cy'umweru gishongesha mu mazi kandi kigakora ifuro rikomeye kandi rihamye iyo rivungaguritse. SDS izwiho ubushobozi bwo gushonga no gushonga poroteyine, bigatuma igira akamaro mu gukuramo poroteyine, kuyisukura no kuyikoresha mu buryo bwa electrophoresis. Byongeye kandi, SDS ikoreshwa cyane nk'isabune mu bikoresho byo kwita ku buzima bw'umuntu mu rugo no mu buzima bwe bwite, ndetse no mu bikorwa byo gusukura mu nganda. Ni kandi ikintu cy'ingenzi mu bushakashatsi bwinshi bwa biochemical na molekile, aho ikoreshwa mu gusesengura uturemangingo, gutandukanya ADN, no nk'ikintu gihindura imiterere y'umubiri muri gel electrophoresis.
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BCA-2K CAS:207124-63-8 Igiciro cy'umukozi
BCA-2K isobanura Beta-Carotene Apocarotenoid-2-Ketolase. Ni enzyme igira uruhare runini mu ikorwa ry’amabara y’ibimera yitwa apocarotenoids. Ayo marabara akomoka kuri molekile ya mbere ya beta-carotene kandi agira uruhare mu mikorere itandukanye y’ibinyabuzima mu bimera.
BCA-2K ihindura by’umwihariko beta-carotene mu buryo bwihariye bw’uturemangingo tw’ibimera binyuze mu kongeramo itsinda rya ketone muri molekile. Iyi enzyme ni ingenzi mu gukora uturemangingo tw’ibimera dufite ibikorwa bitandukanye by’ibinyabuzima n’imikorere yabyo mu bimera.
Byagaragaye ko apocarotenoids ikorwa na BCA-2K igira uruhare mu iterambere ry'ibimera, guhangana n'imihangayiko, no kwirinda indwara n'udukoko. Ishobora kandi gukora nk'ibimenyetso by'uturemangingo tugira uruhare mu mikorere itandukanye y'umubiri.
Inyigo ya BCA-2K n'uruhare rwayo mu gukora apocarotenoids ntabwo ari ingenzi gusa mu gusobanukirwa ibinyabuzima by'ibimera ahubwo inagira akamaro gashoboka mu nzego nko kunoza ibihingwa, korora ibimera, no guteza imbere ibikomoka ku bimera karemano bifite akamaro mu kuvura cyangwa mu mirire.
