N-Ethylmaleimide CAS:128-53-0 Igiciro cy'umukozi

Guhindura Poroteyine: NEM ihindura burundu ibisigazwa bya cysteine ​​muri poroteyine binyuze mu gukora covalent adduct. Uku guhindura bishobora kugira ingaruka ku mikorere ya poroteyine, imiterere, n'imikoranire. Uburyo NEM ikorana n'amatsinda ya sulfhydryl bituma iba igikoresho cy'ingirakamaro mu kwiga imikorere ya poroteyine n'imikorere ishingiye kuri thiol.

Kubuza Enzyme: Enzyme nyinshi zishingira ku matsinda ya thiol kugira ngo zikore neza. Mu gukorana n'amatsinda ya thiol, NEM ishobora gukumira enzymes runaka mu buryo bwihariye. Ibi byakoreshejwe cyane mu kwiga kinetics ya enzymes, gusobanukirwa uburyo enzymes zikora, no gusuzuma enzymes zishobora gukoreshwa.

Ubushakashatsi ku mikoranire ya poroteyine na poroteyine: NEM ikunze gukoreshwa mu gusuzuma imikoranire ya poroteyine na poroteyine. Mu guhagarika ibisigazwa bya tsisteine ​​bihinduka, NEM irinda ishyirwaho ry'imigozi ya disulfide hagati ya poroteyine zikorana, bityo bigahungabanya imikoranire yazo. Ibi bituma abashakashatsi biga uruhare rwa tsisteine ​​runaka mu mikoranire ya poroteyine na poroteyine.

Inyigo ku igenzura rya Redox: NEM ikoreshwa mu kwiga uburyo bwo kugenzura redox muri poroteyine. Mu guhindura cysteine ​​zikora neza, NEM ishobora gushimangira imiterere ya poroteyine runaka no gukumira oxidation idakenewe cyangwa kugabanya ibisigazwa bya cysteine. Ibi bifasha gusobanukirwa uruhare rw'ibimenyetso bya redox n'inzira zo kugenzura redox.

Ubushakashatsi ku guhuza: NEM ishobora gukoreshwa nk'ikintu gihuza poroteyine kugira ngo ihamye poroteyine cyangwa poroteyine zifatanye. Mu guhindura ibisigazwa bya cysteine, NEM ishobora gukora imikoranire hagati ya poroteyine zikorana, bigatuma abashakashatsi bakora ubushakashatsi ku misonge ya poroteyine n'imiterere yayo. Ubu buryo ni ingirakamaro cyane cyane mu bushakashatsi ku miterere y'ibinyabuzima n'imiterere ya poroteyine.

Kuvumbura Imiti: Bitewe n'ubushobozi bwayo bwo gukorana n'ibisigazwa bya cysteine ​​mu buryo bwihariye, NEM yakoreshejwe mu bushakashatsi ku kuvumbura imiti. Ishobora gukoreshwa mu kumenya no kugaragaza aho imiti ishobora kuba igamije cyangwa kwiga umwihariko w'imiti ishobora gukoreshwa ku poroteyine zikungahaye kuri cysteine.