5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide umunyu wa cyclohexylammonium CAS: 18656-96-7 Ishusho y'umwihariko

5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium umunyu CAS: 18656-96-7

5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide Umunyu wa cyclohexylammonium, uzwi kandi ku izina rya X-glucuronide, ni ikintu gikoreshwa mu bushakashatsi bwa biology ya molekile na enzymology. Ni substrate ya chromogenic ikunze gukoreshwa mu kumenya imikorere ya enzyme beta-D-glucuronidase.

Iyi mvange igizwe n'itsinda rya indole rihujwe n'igice cya aside glucuronic binyuze mu mugozi wa glycosidic. Bromo na chloro bisimbura ibinyabutabire bitanga ituze kandi byongera ubushobozi bwo gushonga kw'iyi mvange.

Iyo ishyizwe mu gipimo kirimo beta-D-glucuronidase, enzyme igabanya umugozi wa glycosidic, irekura igice cya indole. Iyi reaction ituma habaho ihinduka ry'ubururu cyangwa ibara, bigatuma byoroshye kubona imikorere ya enzyme mu buryo bw'amaso cyangwa spectrophotometric.

Bitewe n'uburyo ikoresha beta-D-glucuronidase mu buryo bwihariye, X-glucuronide ikoreshwa cyane mu buhanga bwa molekile nka bagiteri n'impinduka z'umusemburo, isuzuma rya gene, no gusiga irangi rya histochemical. Itanga uburyo bworoshye kandi bworoshye bwo kumenya niba beta-D-glucuronidase ihari cyangwa igaragara, kandi ikunze gukoreshwa hamwe n'ibindi bipimo bya gene cyangwa biochemical.

Gutumiza cyangwa Gusaba Igiciro

Ibisobanuro birambuye ku gicuruzwa

Ibirango by'ibicuruzwa

Ishyirwa mu bikorwa n'ingaruka

Ingaruka za 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt (X-glucuronide) ni ubushobozi bwayo bwo gukora ihinduka ry'ubururu cyangwa ibara iyo hari enzyme beta-D-glucuronidase. Iyi enzyme ibaho iyo umugozi wa glycosidic uri hagati y'itsinda rya indole n'igice cya aside glucuronic ugabanutseho na beta-D-glucuronidase.

Gukoresha X-glucuronide ahanini bikorwa mu bushakashatsi bwa molekile na enzymology. Bikunze gukoreshwa nk'ishingiro ryo kumenya imikorere ya beta-D-glucuronidase. Harimo zimwe mu ngamba zihariye zirimo:

Isuzuma rya gene y'umunyamakuru: X-glucuronide ikunze gukoreshwa nk'igice cy'ingenzi mu isuzuma rya gene y'umunyamakuru aho beta-D-glucuronidase ikoreshwa nk'inzobere y'umunyamakuru. Mu guhuza agace k'umutezi cyangwa guhuza urukurikirane rw'ingirabuzimafatizo z'ingenzi kuri gene ya beta-D-glucuronidase, abashakashatsi bashobora gupima imikorere cyangwa imikorere ya gene bakurikirana ikorwa ry'ubururu buto cyangwa impinduka z'ibara iyo X-glucuronide yongewemo.

Isuzuma ry’impinduka za bagiteri n’umusemburo: X-glucuronide ishobora gukoreshwa mu kumenya impinduka nziza za bagiteri cyangwa umusemburo hakoreshejwe vector irimo gene ya beta-D-glucuronidase. Uturemangingo twahinduwe tugaragaza iyo enzyme dushobora guhisha X-glucuronide, bigatuma ibara rihinduka cyangwa rigahinduka.

Gusiga irangi rya histochemical: X-glucuronide ikoreshwa mu buryo bwo gusiga irangi rya histochemical kugira ngo harebwe niba hari kandi ikwirakwira ry’ibikorwa bya beta-D-glucuronidase mu turemangingo cyangwa mu turemangingo. Iri rangi rituma uturemangingo tugaragaza iyo enzyme tubamo kandi tukamenya aho tugeze.

Gupakira ibicuruzwa:

Amakuru y'inyongera:

Imiterere	C26H36BrClN2O7
Isuzuma	99%
Isura	Ifu y'umweru
Nimero ya CAS	18656-96-7
Gupakira	Bito kandi byinshi
Igihe cyo kuruhuka	Imyaka 2
Ububiko	Bika ahantu hakonje kandi humutse
Icyemezo	ISO.