8-Anilino-1-aside naphthalenesulfonike CAS: 82-76-8

8-Anilino-1-naphthalenesulfonic aside, izwi kandi nka ANS, ni irangi ry’urumuri rikunze gukoreshwa mu bushakashatsi bwa biochemical na biophysical. Riri mu bwoko bw’ibinyabutabire by’umwimerere bizwi nka aside sulfonic zishingiye kuri aniline. ANS izwiho ubushobozi bwo kwihuza n’uturere duto duto cyangwa ahantu hagaragaramo hydrophobic muri poroteyine n’izindi biomolecules.

ANS irahinduka iyo ifatanye n'uturere duto duto duto, bigatuma iba igikoresho cy'ingirakamaro cyo kwiga impinduka mu miterere ya poroteyine, imikoranire ya poroteyine na poroteyine, n'uburyo poroteyine zigenda zipfunyika/zigasohoka. Guhuza ANS n'uturere duto duto duto bituma habaho impinduka y'ubururu mu buryo bw'ibyuka bihumanya, bishobora gukurikiranwa hakoreshejwe spectroscopy ya fluorescence.

ANS ikunze gukoreshwa mu buryo butandukanye bw'ubushakashatsi, harimo fluorescence polarization, fluorescence resonance energy transfer (FRET), no gusuzuma ubushyuhe. Yakoreshejwe mu kwiga imiterere n'ubudahangarwa bwa poroteyine, imikoranire ya poroteyine na ligand, hamwe n'uburyo poroteyine ziteranywa.

Gutumiza cyangwa Gusaba Igiciro

Ibisobanuro birambuye ku gicuruzwa

Ibirango by'ibicuruzwa

Ishyirwa mu bikorwa n'ingaruka

Icupa ry'urumuri: ANS ikoreshwa cyane nk'icupa ry'urumuri mu kwiga impinduka mu miterere ya poroteyine, inzira zo gupfunyika/gusohora poroteyine, n'imikoranire ya poroteyine na ligand. Ifatana n'uturere twa hydrophobic cyangwa ahantu hagaragara hydrophobic muri poroteyine, bigatuma habaho ibyuka bya fluorescence bishobora gukurikiranwa hakoreshejwe spectroscopy ya fluorescence.

Gupima ubukana bw'amazi: ANS ibasha kumenya impinduka mu bukana bw'amazi mu bidukikije. Ishobora gukoreshwa mu kumenya no gupima surfactants, sabuni, n'ibindi bintu birwanya ubukana bw'amazi.

Isuzuma ry’ubudahangarwa bwa poroteyine: ANS ikunze gukoreshwa mu isuzuma ry’ubudahangarwa bw’ubushyuhe kugira ngo isuzume ubudahangarwa bwa poroteyine. Mu kugenzura ubukana bwa poroteyine ziboheshejwe na ANS mu bushyuhe butandukanye, birashoboka kumenya imiterere y’ubudahangarwa bw’ubushyuhe n’ubushyuhe bwo gushonga (Tm) bya poroteyine.

Inyigo ku guteranya poroteyine: ANS ishobora gukoreshwa mu kwiga inzira zo guteranya poroteyine, nko kurema fibrils za amyloid. Irangi rishobora kwitabwaho n'uturere twagaragayemo hydrophobic mu guteranya poroteyine, bigatuma riba igikoresho cy'ingirakamaro mu gukurikirana iterambere n'imikorere y'iteranya.

Imiterere y'imikoranire ya poroteyine na poroteyine: ANS yakoreshejwe mu gusuzuma imikoranire ya poroteyine na poroteyine. Iyo poroteyine zihura, hashobora kubaho impinduka mu bijyanye no kudakoresha amazi, ibyo bikaba byagenzurwa hakoreshejwe fluorescence ya ANS.

Kuvumbura no guteza imbere imiti: ANS ishobora gukoreshwa mu gusuzuma imiti ishobora gukoreshwa. Ishobora gufasha mu gusuzuma uburyo molecule nto zifatanya n'imibanire yazo na poroteyine, bigatuma abashakashatsi bamenya ibintu bishobora guhindura imikorere ya poroteyine.

Gupakira ibicuruzwa:

Amakuru y'inyongera:

Imiterere	C16H13NO3S
Isuzuma	99%
Isura	Ifu y'icyatsi kibisi cyijimye
Nimero ya CAS	82-76-8
Gupakira	Bito kandi byinshi
Igihe cyo kuruhuka	Imyaka 2
Ububiko	Bika ahantu hakonje kandi humutse
Icyemezo	ISO.