Nitrotetrazolium blue chloride CAS:298-83-9

Nitrotetrazolium blue chloride (NBT) ni irangi rikoreshwa cyane mu gusuzuma ibinyabuzima n'ibikomoka kuri biokemike. Rikoreshwa cyane cyane nk'ikimenyetso cya redox kugira ngo harebwe kandi hagaragazwe niba hari enzymes za dehydrogenase mu bipimo by'ibinyabuzima.

Iyo NBT igabanutse bitewe na enzymes za dehydrogenase, ihinduka ibara kuva ku muhondo werurutse ikajya ku bururu bwijimye bwa formazan. Iri hinduka rituma abashakashatsi bamenya imikorere n'aho enzymes za dehydrogenase ziherereye mu turemangingo n'uturemangingo dutandukanye. Kubera iyo mpamvu, NBT ikunze gukoreshwa mu gusuzuma no mu bushakashatsi ku birebana n'ubudahangarwa bw'umubiri kugira ngo hamenyekane imikorere y'uturemangingo n'imikorere ya enzymes.

Mu gupima ubukana bw'ibara ry'ubururu ryakozwe, abashakashatsi bashobora gusesengura imikorere ya enzyme ya dehydrogenase mu buryo bw'imibare, ibyo bikaba ari ingenzi mu gusobanukirwa imikorere y'uturemangingo nko guhangayika kwa oxidative, guhumeka kw'uturemangingo, no guhinduka kwa karubohidrati na lipide. Aya makuru ni ingenzi mu nzego zitandukanye, harimo biochimie, biology y'uturemangingo, no gusuzuma indwara.