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PNPG CAS: 3150-24-1 Igiciro cyabakora
PNPG, cyangwa p-nitrophenyl β-D-glucopyranoside, ni substrate ntoya ya molekile ikunze gukoreshwa mubushakashatsi bwibinyabuzima kugirango bapime ibikorwa byimisemburo ya glucosidase.Ntabwo ifite ibara kandi idafite fluorescent, ariko kuri hydrolysis na glucosidase, ihinduka p-nitropenol, ifite ibara ry'umuhondo kandi ikaboneka byoroshye kuri spekitifoto.
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URUBUGA RWA ONPG: 369-07-3 Igiciro cyabakora
O-nitrophenyl-β-D-galactopyranoside (ONPG) ni insimburangingo ikoreshwa mu binyabuzima bya biohimiki na molekuline yerekana gupima ibikorwa bya enzyme β-galactosidase.Bikunze gukoreshwa mubisubizo kugirango hamenyekane imvugo ya gene muri sisitemu ya bagiteri, nka Escherichia coli.ONPG nuruvange rutagira ibara ruvanze na β-galactosidase, bikavamo irekurwa ryumuhondo, o-nitrophenol.Ibara ry'umuhondo ryakozwe rishobora gupimwa spekitifotometometricique, ritanga igipimo kiziguye cyibikorwa bya enzyme.Ikigereranyo ukoresheje ONPG bakunze kwita ONPG kandi ni uburyo bukoreshwa cyane mubushakashatsi bwibinyabuzima bwa molekuline kugirango hamenyekane urwego rwimiterere ya gen igenzurwa na lac. operon muri selile.
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Nitrotetrazolium Ubururu bwa Chloride CAS: 298-83-9
Nitrotetrazolium Ubururu bwa Chloride (NBT) ni ikimenyetso cya redox gikunze gukoreshwa mubushakashatsi bwibinyabuzima na biohimiki.Nifu yumuhondo yijimye ihinduka ubururu iyo igabanijwe, bigatuma iba ingirakamaro mugutahura ko hari imisemburo imwe nimwe nibikorwa bya metabolike.
NBT ikorana na electron hamwe na enzymes nka dehydrogenase, bigira uruhare mubikorwa bitandukanye bya selile.Iyo NBT igabanijwe niyi misemburo, ikora imvura yubururu bwa formazan, itanga uburyo bwo kumenya cyangwa kureba ibintu.
Iyi reagent isanzwe ikoreshwa mubisubizo nkikizamini cyo kugabanya NBT, aho ikoreshwa mugusuzuma ibikorwa byubuhumekero biturika byingirabuzimafatizo.Irashobora kandi gukoreshwa mukwiga ibikorwa bya enzyme ninzira ya metabolike mubushakashatsi bujyanye na stress ya okiside, viabilite selile, no gutandukanya selile.
NBT yabonye porogaramu mubice bitandukanye, harimo microbiologiya, immunologiya, na biologiya selile.Iratandukanye, irasa neza, kandi yoroshye kuyikoresha, bituma ihitamo gukundwa na protocole nyinshi zigerageza.
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Neocuproine CAS: 484-11-7 Igiciro cyabakora
Neocuproine ni chelating agent ikunze gukoreshwa mubikorwa bitandukanye, harimo chimie yisesengura na farumasi.Ifite isano ikomeye kuri ion z'umuringa kandi ikora ibintu bihamye hamwe nayo.Uyu mutungo utuma neocuproine igira akamaro mugushakisha no kugereranya umuringa mubisubizo cyangwa ingero.Byongeye kandi, neocuproine yakozweho ubushakashatsi ku buryo ishobora kuvura, cyane cyane mu kuvura kanseri n'indwara zifata ubwonko.
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IPTG CAS: 367-93-1 Igiciro cyabakora
Isopropyl β-D-1-thiogalactopyranoside (IPTG) ni ikigereranyo cya synthique ya lactose ikoreshwa cyane mubushakashatsi bwibinyabuzima bya molekuline no gukoresha ibinyabuzima.IPTG ikoreshwa cyane cyane mugukangura imiterere ya gen muri sisitemu ya bagiteri, aho ikora nka molekuline yo gutangiza iyandikwa rya gen.
Iyo wongeyeho uburyo bwo gukura, IPTG ifatwa na bagiteri kandi irashobora guhuza na proteine ya lac repressor, ikabuza guhagarika ibikorwa bya lac operon.Lac operon ni ihuriro rya gen zigira uruhare muri metabolism ya lactose, kandi iyo poroteyine ya repressor ikuweho, gen zigaragazwa.
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CAS HATU: 148893-10-1 Igiciro cyabakora
HATU (1- [bis (dimethylamino) methylene] -1H-1,2,3-triazolo
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D-fucose CAS: 3615-37-0 Igiciro cyabakora
D-fucose ni monosaccharide, cyane cyane isukari itandatu ya karubone, iri mumatsinda yisukari yoroshye yitwa hexose.Ni isomer ya glucose, itandukanye muburyo bw'itsinda rimwe rya hydroxyl.
D-fucose isanzwe iboneka mubinyabuzima bitandukanye, harimo bagiteri, ibihumyo, ibimera, ninyamaswa.Ifite uruhare runini mubikorwa byinshi byibinyabuzima, nkibimenyetso byerekana ingirabuzimafatizo, ingirabuzimafatizo, hamwe na synthesis ya glycoproteine.Nibigize glycolipide, glycoproteine, na proteoglycans, bigira uruhare mu itumanaho rya selile-selile no kumenyekana.
Mu bantu, D-fucose igira kandi uruhare muri biosynthesis yimiterere yingenzi ya glycan, nka Lewis antigens na antigens groupe yamaraso, bigira uruhare muburyo bwo guhuza amaraso no kwandura indwara.
D-fucose irashobora kuboneka ahantu hatandukanye, harimo ibyatsi byo mu nyanja, ibimera, na fermentation ya mikorobe.Ikoreshwa mubushakashatsi no gukoresha biomedical medicine, ndetse no mugukora imiti imwe nimwe yimiti hamwe nubuvuzi.
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DDT CAS: 3483-12-3 Igiciro cyabakora
DL-Dithiothreitol, izwi kandi ku izina rya DTT, ni ikintu kigabanya cyane gikoreshwa mu bushakashatsi bw’ibinyabuzima na biologiya.Ni molekile ntoya ifite itsinda rya thiol (irimo sulfure) kuri buri mpera.
DTT ikoreshwa kenshi kugirango isibe disulfide ihuza poroteyine, ifasha guhishura cyangwa kuyitandukanya.Uku kugabanya imiyoboro ya disulfide ni ngombwa muburyo butandukanye bwa laboratoire nko kweza poroteyine, gel electrophorei, hamwe nubushakashatsi bwa protein.DTT irashobora kandi gukoreshwa mukurinda amatsinda ya thiol no kwirinda okiside mugihe cyibigeragezo.
DTT isanzwe yongerwaho ibisubizo byubushakashatsi mubitekerezo bito, kandi ibikorwa byayo biterwa no kuba ogisijeni ihari.Ni ngombwa gufata neza DTT kuko yunvikana ikirere, ubushyuhe, nubushuhe, bishobora kugabanya imikorere yabyo.
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D - (+) - Galactose CAS: 59-23-4 Igiciro cyabakora
D - (+) - Galactose ni isukari ya monosaccharide kandi nikintu cyingenzi cyibinyabuzima byinshi.Nisukari isanzwe iboneka mubiribwa byinshi, nk'imbuto, ibikomoka ku mata, n'imboga.
Galactose isanzwe ihindagurika mumubiri binyuze murukurikirane rwimikorere.Ifite uruhare runini mu itumanaho rya selile, kubyara ingufu, hamwe na biosynthesis ya molekile zikomeye nka glycolipide, glycoproteine, na lactose.
Kubireba ikoreshwa ryayo, D - (+) - Galactose ikoreshwa muri mikorobiologiya na biotechnologiya nkisoko ya karubone mubitangazamakuru byumuco kugirango ikure mikorobe zitandukanye.Irakoreshwa kandi mukubyara ibinyabuzima bitandukanye, imiti, nibiribwa.Byongeye kandi, ikoreshwa kenshi nk'umukozi wo gusuzuma indwara, cyane cyane mu bizamini byo gusuzuma imikorere y'umwijima no kumenya indwara zishingiye ku ngirabuzima fatizo zijyanye na metabolism ya galaktose.
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beta-D-Galactose pentaacetate CAS: 4163-60-4
Beta-D-Galactose pentaacetate ni imiti ivangwa na galactose, isukari ya monosaccharide.Ikorwa na acetylating buri hydroxyl groupe ya molekile ya galactose hamwe na acetyl eshanu.
Uru ruganda rukunze gukoreshwa nkibikoresho birinda galactose muburyo butandukanye bwimiti nuburyo bukoreshwa.Ifishi ya pentaacetate ifasha guhagarika galactose no gukumira ibisubizo bidakenewe cyangwa guhinduka mugihe cya reaction.
Byongeye kandi, iyi nteruro irashobora gukoreshwa nkibibanziriza synthesis yizindi nkomoko ya galaktose.Amatsinda ya acetyl arashobora gukurwaho kugirango abone ibikomoka kuri galaktose bitandukanye hamwe nitsinda ryimikorere.
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5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide umunyu wa sodium CAS: 129541-41-9
5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide umunyu wa sodium ni uruganda rwimiti rukoreshwa mubushakashatsi bwa laboratoire no gusuzuma.Bikunze kwitwa X-Gluc kandi ikoreshwa cyane nka substrate yo kumenya ibikorwa bya enzyme ya beta-glucuronidase.
Iyo beta-glucuronidase ihari, ikuraho glucuronide muri X-Gluc, bikavamo kwibohora irangi ry'ubururu ryitwa 5-bromo-4-chloro-3-indolyl.Iyi reaction isanzwe ikoreshwa muburyo bwo kureba cyangwa kwerekana spekitifotometometrike kugirango igaragaze imvugo ya enzyme ya beta-glucuronidase muri selile cyangwa tissue.
Umunyu wa sodium ya X-Gluc utezimbere gukemura ibibazo byamazi, byoroshya gukoresha muri laboratoire.X-Gluc ikoreshwa cyane mubushakashatsi bwibinyabuzima bwa molekuline kugirango bige imvugo ya gene, ibikorwa byabateza imbere, hamwe numunyamakuru gene avuga.Irashobora kandi gukoreshwa kugirango hamenyekane ko ibinyabuzima bitanga beta-glucuronidase, nka bagiteri zimwe na zimwe, mu bushakashatsi bwa mikorobi.
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4-Nitrophenyl-beta-D-xylopyranoside CAS: 2001-96-9
4-Nitrophenyl-beta-D-xylopyranoside ni chromogenic substrate ikoreshwa mubushakashatsi bwimisemburo kugirango tumenye kandi dupime ibikorwa byimisemburo yitwa beta-xylosidase.
