DDT CAS:3483-12-3 Igiciro cy'umukozi

DL-Dithiothreitol, izwi kandi nka DTT, ni ikintu gifasha mu kugabanya ubukana bw'ibinyabutabire n'ibinyabuzima. Ni molekile nto ifite itsinda rya thiol (ririmo sulfur) ku mpera zose.

DTT ikunze gukoreshwa mu guca imigozi ya disulfide muri poroteyine, ibyo bigafasha kuyikura cyangwa kuyihindura. Uku kugabanya imigozi ya disulfide ni ingenzi mu buryo butandukanye bwo muri laboratwari nko gusukura poroteyine, electrophoresis ya gel, no kwiga imiterere ya poroteyine. DTT ishobora kandi gukoreshwa mu kurinda amatsinda ya thiol no gukumira oxidation mu gihe cy'igerageza.

DTT ikunze kongerwa mu bisubizo by'igerageza mu rugero ruto, kandi imikorere yayo iterwa n'uko umwuka wa ogisijeni uhari. Ni ngombwa kuyifata neza kuko ishobora kwangirika n'umwuka, ubushyuhe n'ubushuhe, ibyo bikaba bishobora kugabanya ubushobozi bwayo.

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Kugabanya imigozi ya Disulfide: DTT ikoreshwa cyane cyane mu guca imigozi ya disulfide, ari yo migozi ihuza ibisigazwa bibiri bya cysteine muri poroteyine. Mu kugabanya iyi migozi, DTT ifasha guhindura poroteyine, bigatuma isesengura imiterere yazo n'imikorere yazo rirushaho kuba ryiza.

Kuzingira Poroteyine: DTT ishobora gufasha mu kuzingira neza poroteyine mu gukumira ishyirwaho ry’imigozi ya disulfide ritari ryo. Igabanya imigozi ya disulfide itari iy’umwimerere ishobora kubaho mu gihe poroteyine izingira, bigatuma poroteyine ihinduka nk’uko isanzwe imeze.

Igikorwa cya Enzyme: DTT ishobora gukora enzymes zimwe na zimwe igabanya imigozi iyo ari yo yose ibuza disulfide kuba iriho. Byongeye kandi, DTT ishobora gukumira oxidation y'ibisigazwa by'ingenzi bya cysteine, bishobora kuba ngombwa kugira ngo enzyme ikore.

Gukora Antibody: DTT ikunze kongerwamo kugira ngo igabanye imigozi ya disulfide mu gihe cyo gukora antibody. Ifasha mu gukumira ikorwa ry’imigozi ya disulfide itari yo, ishobora kubangamira imigozi ikwiye ya antigen.

Gutuza Poroteyine: DTT ishobora gukoreshwa mu gutuza poroteyine mu gukumira ogisijeni cyangwa kwishyira hamwe kwazo. Ifasha mu kubungabunga imiterere ya poroteyine mu gihe cyo kubika no gukora igerageza.

Ibintu Bigabanya Ibice mu Bumenyi bw'ibinyabuzima: DTT ikunze gukoreshwa mu buryo butandukanye bwa biology ya molecule nko gusesengura ADN, PCR, no gutunganya poroteyine. Ishobora gufasha kugumana imiterere igabanuka y'ibice by'ingenzi, bigatuma habaho ibisubizo byiza ku igerageza.

Urugero rw'ibicuruzwa

Gupakira ibicuruzwa:

Amakuru y'inyongera:

Imiterere	C4H10O2S2
Isuzuma	99%
Isura	Ifu y'umweru
Nimero ya CAS	3483-12-3
Gupakira	Bito kandi byinshi
Igihe cyo Kubika	Imyaka 2
Ububiko	Bika ahantu hakonje kandi humutse
Icyemezo	ISO.