Nitrotetrazolium Blue Chloride CAS: 298-83-9

Nitrotetrazolium Blue Chloride (NBT) ni ikimenyetso cya redox gikunze gukoreshwa mu isuzuma ry’ibinyabuzima n’ibijyanye na biochemical. Ni ifu y’umuhondo werurutse ihinduka ubururu iyo yagabanutse, bigatuma iba ingirakamaro mu kumenya niba hari enzymes zimwe na zimwe n’imikorere ya metabolism.

Ingaruka nyamukuru za NBT ni ukurema ubururu bwa formazan iyo bugabanutse bitewe na enzymes zimwe na zimwe. Uku guhinduka kw'amabara bituma habaho kumenya imikorere ya enzyme mu buryo bw'amaso cyangwa bwa spectrophotometric.

NBT ifite uburyo butandukanye bwo kuyikoresha mu bushakashatsi no mu gusuzuma indwara. Dore zimwe mu nzira zayo z'ibanze:

Isuzuma ry'imikorere ya enzyme: NBT ishobora gukoreshwa mu gupima imikorere ya dehydrogenases, igira uruhare mu bikorwa nko guhumeka kw'uturemangingo no mu mikorere y'uturemangingo. Mu kugenzura igabanuka rya NBT muri formazan, abashakashatsi bashobora gusuzuma imikorere y'izi enzymes.

Isuzuma ry'imikorere y'uturemangingo tw'ubudahangarwa bw'umubiri: NBT ikoreshwa cyane mu isuzuma rya NBT kugira ngo harebwe imikorere y'uturemangingo tw'ubudahangarwa bw'umubiri, cyane cyane phagocytes. Isuzuma ripima ubushobozi bw'utu turemangingo bwo gukora ubwoko bwa ogisijeni bushobora kugabanya NBT no gukora ubururu.

Ubushakashatsi ku binyabuzima: NBT ikoreshwa muri binyabuzima mu kwiga imikorere ya mikorobe no gusuzuma imikorere ya enzymes runaka. Urugero, yakoreshejwe mu gupima bagiteri nitrate reductases cyangwa bagiteri zikora formazan.

Inyigo ku buzima bw'uturemangingo: Kugabanya NBT bituma abashakashatsi basuzuma imikorere ya metabolike n'ubuzima bw'uturemangingo. Mu gupima ubukana bw'umusaruro w'ubururu wa formazan, birashoboka kumenya umubare w'uturemangingo dushobora kubaho mu rugero runaka.