ONPG CAS:369-07-3 Igiciro cy'uruganda

Ingaruka za ONPG nk'igice cy'umubiri zigomba gucibwamo na enzyme β-galactosidase, bigatuma habaho irekurwa ry'umuhondo, o-nitrophenol. Iri hinduka ry'ibara rishobora gupimwa hifashishijwe spectrophotometric, bigatuma hagaragazwa imikorere ya β-galactosidase. Gukoresha ONPG ahanini ni mu gusuzuma imikorere ya gene mu bushakashatsi bwa molekile na mikorobiyoloji. Ikoreshwa cyane mu gupima imikorere ya β-galactosidase nk'umunyamakuru w'inyigo z'imikorere ya gene, cyane cyane muri bagiteri nka E. coli. Gene ya lacZ, ikora β-galactosidase, ikunze gukoreshwa nk'ikimenyetso cyo gusesengura imikorere ya gene, kuko imikorere yayo ishobora guterwa n'imimerere runaka cyangwa ikagenzurwa n'abayikora. Isuzuma rya ONPG ritanga uburyo bworoshye kandi bwizewe bwo gusuzuma urwego rw'imikorere ya gene hapimwa imikorere ya β-galactosidase. Iri suzuma rikoreshwa cyane mu bikorwa bitandukanye, nko kwiga imikorere ya promoter, kugenzura imiterere ya gene, no gukorana kwa poroteyine na poroteyine. Byongeye kandi, ishobora gukoreshwa mu kumenya ikinyabutabire cya enzyme no gusuzuma ingaruka z'impinduka cyangwa uburyo bwo kuvura ku mikorere ya enzyme.