5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide umunyu wa sodium CAS: 129541-41-9

Umunyu wa sodium wa 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide ni ikinyabutabire gikoreshwa cyane mu bushakashatsi no mu gusuzuma indwara muri laboratwari. Akenshi witwa X-Gluc kandi ukoreshwa cyane nk'ikintu cyo gupima imikorere ya enzyme ya beta-glucuronidase.

Iyo beta-glucuronidase ihari, igabanya umugozi wa glucuronide muri X-Gluc, bigatuma irangi ry'ubururu ryitwa 5-bromo-4-chloro-3-indolyl risohoka. Iyi reaction ikunze gukoreshwa mu kumenya mu buryo bw'amaso cyangwa spectrophotometric imikorere ya enzyme ya beta-glucuronidase mu turemangingo cyangwa mu turemangingo.

Umunyu wa sodiyumu wa X-Gluc utuma ishonga mu mazi irushaho gushonga, bityo ikorohereza ikoreshwa ryayo mu isuzuma rya laboratwari. X-Gluc ikoreshwa cyane cyane mu bushakashatsi ku binyabuzima bya molekile kugira ngo yige imiterere y’uturemangingo, imikorere ya promoter, n’isuzuma rya reporter gene. Ishobora kandi gukoreshwa mu gusuzuma ko hari utunyabuzima dukora beta-glucuronidase, nka bagiteri zimwe na zimwe, mu bushakashatsi bwa mikorobe.

Gutumiza cyangwa Gusaba Igiciro

Ibisobanuro birambuye ku gicuruzwa

Ibirango by'ibicuruzwa

Ishyirwa mu bikorwa n'ingaruka

Gupima GUS: Gluc ya X igabanywamo na enzyme ya GUS ikayishyiramo ikintu cy'ubururu kidashonga kizwi nka 5-bromo-4-chloro-3-indole (X-Ind). Iki gikorwa gituma habaho kureba no gupima imikorere ya GUS mu turemangingo no mu ngingo.

Inyigo z’igaragara ry’uturemangingo: X-Gluc ikoreshwa nk'umunyamakuru mu inyigo z’igaragara ry’uturemangingo. Mu guhuza gene ya GUS n'ikintu gishishikaza, abashakashatsi bashobora kumenya imikorere n'imiterere y'igaragara ry'uturemangingo binyuze mu kumenya imikorere ya GUS bakoresheje X-Gluc.

Isesengura ry’ibimera byahinduwe mu jini: Sisitemu ya GUS reporter gene ikoreshwa cyane mu bimera bya molekile. Gusiga irangi rya X-Gluc bituma abashakashatsi bashobora kubona no kwiga imiterere y’igaragara rya transgene mu bimera. Ibi bifasha gusobanukirwa uburyo jini zigenga, imiterere yihariye y’ingingo, n’imiterere y’iterambere ry’ibimera.

Ubwubatsi bw'uturemangingo: X-Gluc ikoreshwa nk'ikimenyetso gishobora gutoranywa mu bushakashatsi bw'ubuhanga bw'uturemangingo. Mu guhuza gene ya GUS na gene yo mu mahanga, gusiga irangi rya X-Gluc bishobora gukoreshwa mu kumenya impinduka nziza no guhuza gene zifuzwa mu binyabuzima.

Ubushakashatsi ku mikorobe: X-Gluc ishobora gukoreshwa mu gupima no kumenya bagiteri zikora GUS. Iyi enzyme GUS iboneka mu moko menshi atandukanye ya bagiteri, kandi gusiga irangi kuri X-Gluc bituma habaho kureba no kumenya bagiteri za GUS mu bushakashatsi bwa mikorobe.

Urugero rw'ibicuruzwa

Gupakira ibicuruzwa:

Amakuru y'inyongera:

Imiterere	C14H14BrClNNaO7
Isuzuma	99%
Isura	Ifu y'umweru
Nimero ya CAS	129541-41-9
Gupakira	Bito kandi byinshi
Igihe cyo Kubika	Imyaka 2
Ububiko	Bika ahantu hakonje kandi humutse
Icyemezo	ISO.