aside pentaacetic ya diethylene triamine CAS: 67-43-6

Guhagarika RNases: Intego y'ibanze ya DEPC ni uguhagarika RNases, ari zo enzymes zishobora kwangiza RNA. DEPC ikorana n'ibisigazwa bya histidine muri RNases, igahindura ahantu hakora kandi bigatuma bidakora. Ibi bishimangira ko RNA igumana ubusugire bwayo mu gihe cy'igerageza nko gutandukanya RNA, guhindura inyandiko, no gukurikirana RNA.

Kubika ingero za RNA: Ubuvuzi bwa DEPC bukunze gukoreshwa ku bikoresho bya laboratwari, nk'imiyoboro ya microcentrifuge, imiyoboro ya pipette, n'amazi akoreshwa mu igerageza rya RNA. Ibi bifasha gukumira RNases zisigaye zishobora kuba zihari no gukumira kwangirika kwa RNA mu gihe cyo gufata no kubika ingero.

Gutegura reagent idafite RNAse: DEPC ishobora gukoreshwa mu kuvura ibisubizo cyangwa reagents zigomba kuba zidafite RNAse, nka buffers, Tris-EDTA (TE), cyangwa amazi akoreshwa mu bushakashatsi bwa molekile. Iyi reagents yavuwe ishobora gukoreshwa mu buryo bwizewe kugira ngo igabanye kwangirika kwa RNA.

Guhindura ADN: DEPC ishobora kandi gukorana n'amatsinda ya amino muri molekile za ADN, bigatuma habaho guhindura karbetoxylated kuri ADN bases. Ibi bishobora gukoreshwa mu buryo bwihariye, nko gukora ADN sequencing reactions cyangwa guhindura ADN mu bushakashatsi bwo guhuza.