DEPC CAS: 1609-47-8 Igiciro cy'umukozi

Diethyl pyrocarbonate (DEPC) ni ikinyabutabire gikunze gukoreshwa muri laboratwari za molekile. Ni amazi asobanutse kandi adafite ibara, akorana cyane n’ibintu bitera imiterere y’uturemangingo nka amine na thiols. DEPC ikoreshwa cyane cyane mu guhagarika RNases (ribonucleases), enzymes zishobora kwangiza RNA, binyuze mu guhindura aho zikorera. Ibi ibigeraho binyuze mu guhuza n’ibisigazwa bya histidine muri enzyme, ari byo by’ingenzi mu mikorere yayo. Ibi bituma DEPC iba igikoresho cy’agaciro mu bushakashatsi bujyanye na RNA kandi ikanatuma RNA ikomeza kubungabungwa. Uretse imiterere yayo itera imiterere y’uturemangingo, DEPC ishobora no guhindura izindi biomolecules, nka poroteyine na ADN, bitewe nuko ikorana n’amatsinda atera imiterere y’uturemangingo. Hakwiye kwitonderwa mu gihe cyo gufata DEPC, kuko ari uburozi kandi ishobora guhindura imiterere y’uturemangingo.

Gutumiza cyangwa Gusaba Igiciro

Ibisobanuro birambuye ku gicuruzwa

Ibirango by'ibicuruzwa

Ishyirwa mu bikorwa n'ingaruka

Guhagarika RNases: Intego y'ibanze ya DEPC ni uguhagarika RNases, ari zo enzymes zishobora kwangiza RNA. DEPC ikorana n'ibisigazwa bya histidine muri RNases, igahindura ahantu hakora kandi bigatuma bidakora. Ibi bishimangira ko RNA igumana ubusugire bwayo mu gihe cy'igerageza nko gutandukanya RNA, guhindura inyandiko, no gukurikirana RNA.

Kubika ingero za RNA: Ubuvuzi bwa DEPC bukunze gukoreshwa ku bikoresho bya laboratwari, nk'imiyoboro ya microcentrifuge, imiyoboro ya pipette, n'amazi akoreshwa mu igerageza rya RNA. Ibi bifasha gukumira RNases zisigaye zishobora kuba zihari no gukumira kwangirika kwa RNA mu gihe cyo gufata no kubika ingero.

Gutegura reagent idafite RNAse: DEPC ishobora gukoreshwa mu kuvura ibisubizo cyangwa reagents zigomba kuba zidafite RNAse, nka buffers, Tris-EDTA (TE), cyangwa amazi akoreshwa mu bushakashatsi bwa molekile. Iyi reagents yavuwe ishobora gukoreshwa mu buryo bwizewe kugira ngo igabanye kwangirika kwa RNA.

Guhindura ADN: DEPC ishobora kandi gukorana n'amatsinda ya amino muri molekile za ADN, bigatuma habaho guhindura karbetoxylated kuri ADN bases. Ibi bishobora gukoreshwa mu buryo bwihariye, nko gukora ADN sequencing reactions cyangwa guhindura ADN mu bushakashatsi bwo guhuza.

Gupakira ibicuruzwa:

Amakuru y'inyongera:

Imiterere	C6H10O5
Isuzuma	99%
Isura	Amazi adafite ibara
Nimero ya CAS	1609-47-8
Gupakira	Bito kandi byinshi
Igihe cyo kuruhuka	Imyaka 2
Ububiko	Bika ahantu hakonje kandi humutse
Icyemezo	ISO.