Ethidium Bromide CAS: 1239-45-8

Ethidium bromide (EtBr) ni irangi risanzwe rikoreshwa mu bugenge bw'ibinyabuzima na biochimie mu kureba aside nucleic, cyane cyane ADN, muri agarose gel electrophoresis. Rifite ubufatanye bukomeye na ADN, kandi iyo rishyizwe ku rumuri rwa ultraviolet (UV), ritanga fluoresces kandi rigatanga ibara ry'umutuku n'icunga. Ibi bituma abashakashatsi bashobora kubona no gusesengura byoroshye ibice bya ADN bitandukanyijwe n'ingano muri gel matrix.

EtBr ihuza ibice bibiri by’ibanze bya ADN na RNA, bigatuma habaho impinduka mu miterere yayo ya fluorescence. Ikunze kongerwa muri agarose gels cyangwa ikavangwa n’ibipimo bya ADN mbere yo gukoresha electrophoresis. Iyo gel ihuye n’urumuri rwa UV, uduce twa ADN turimo EtBr tugaragara nk’uduce tw’umuhondo twijimye, bigatuma byoroha kubona no gusesengura uduce twa ADN.

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Ishyirwa mu bikorwa n'ingaruka

Kureba ADN/RNA: EtBr ikoreshwa cyane mu kureba ADN na RNA muri agarose gel electrophoresis. Ihuza hagati y’ibice by’ibanze bya molecules za aside nucleic, bigatuma zihinduka fluoresce iyo zihuye n’urumuri rwa UV. Ibi bituma abashakashatsi bashobora kwiyumvisha no gusesengura itandukanyirizo ry’ibice bya ADN cyangwa RNA bitewe n’ingano yabyo cyangwa urukurikirane rwabyo.

Gusiga irangi rya ADN: EtBr ishobora gukoreshwa mu gusiga irangi rya ADN mu buryo butandukanye bwo kugerageza. Ishobora kongerwa mu bipimo bya ADN, nka plasmide zisukuye cyangwa ibikoresho bya PCR, kugira ngo harebwe niba zihari kandi zifite ubunini bwazo. ADN isigaye ishobora kugaragazwa hakoreshejwe urumuri rwa UV cyangwa sisitemu yo gufata amashusho ya gel.

Ingano ya ADN: EtBr irashobora gukoreshwa mu gupima ingano ya ADN. Mu kugereranya ubukana bwa ADN za EtBr ziri muri gel ya agarose n'ibipimo bizwi by'ingano ya ADN, ingano ya ADN iri mu gipimo ishobora kumenyekana.

Mikorosikopi y'urumuri: EtBr ishobora gukoreshwa muri mikorosikopi y'urumuri kugira ngo irangize kandi igaragaze ADN mu turemangingo cyangwa mu turemangingo tuzima. Ituma abashakashatsi biga aho ADN iherereye n'uburyo ihinduka mu turemangingo cyangwa bakareba uburyo ADN icikamo ibice mu turemangingo.

Gupakira ibicuruzwa:

Amakuru y'inyongera:

Imiterere	C21H20BrN3
Isuzuma	99%
Isura	Ifu itukura kugeza ku ibara ry'umuhengeri wijimye
Nimero ya CAS	1239-45-8
Gupakira	Bito kandi byinshi
Igihe cyo kuruhuka	Imyaka 2
Ububiko	Bika ahantu hakonje kandi humutse
Icyemezo	ISO.