Tris (hydroxymethyl) nitromethane CAS: 126-11-4

Ingaruka:

Ubushobozi bwa Buffering: Tris ikora nkigikorwa cyiza cya buffering bitewe nubushobozi bwayo bwo kwakira cyangwa gutanga proton, kugumana urwego ruhamye rwa pH mubisubizo.Bikunze gukoreshwa nkibice byibanze muri sisitemu ya buffer kugirango ihagarike pH yintangarugero yibinyabuzima n'ibisubizo.

Porogaramu:

Ibinyabuzima bya Molecular: Tris ikoreshwa cyane nkibikoresho byifashishwa mu buhanga butandukanye bw’ibinyabuzima, harimo ADN na RNA kwigunga, PCR, gel electrophorei, no kweza poroteyine.Ifasha kubungabunga ibidukikije pH ihamye, itanga uburyo bwiza bwo gukora enzymatique no guhuza molekile.

Umuco w'akagari: Tris ikoreshwa kenshi mubitangazamakuru byumuco w'akagari kugirango igumane uburinganire bwa pH na osmotic, bufasha gukura neza kwingirabuzimafatizo no kubaho neza.

Ubuhanga bwa poroteyine: Tris ikoreshwa mubushakashatsi bwa chimie proteyine, nka protein solubilisation, proteine ​​zihamye, hamwe nubushakashatsi bwa protein-ligand.Ifasha kugumana urwego rwa pH rwifuzwa, kwemeza poroteyine ikwiye hamwe nibikorwa.

Enzymology: Tris ikoreshwa muburyo butandukanye bwimisemburo kugirango ihindure imiterere ya pH isabwa mubikorwa byimikorere.Itanga ibisubizo byizewe kandi byororoka, bigafasha gupima neza enzyme kinetics hamwe nubushakashatsi bwo kubuza.

Isuzuma ryibinyabuzima: Tris ikoreshwa nkibigize mubice byinshi bya biohimiki bitewe nuburyo bwayo.Ikomeza pH ihoraho mugihe cyamabara, spekitifotometometrike, hamwe na enzymatique isuzuma, byongerera ukuri no kwizerwa kubisubizo.