Tris (hydroxymethyl)nitromethane CAS: 126-11-4

Ingaruka:

Ubushobozi bwo Gufata Ibinure: Tris ikora nk'igikoresho cyiza cyo gufata ibinure bitewe n'ubushobozi bwayo bwo kwakira cyangwa gutanga protoni, igakomeza kugira pH ihamye mu bisubizo. Ikunze gukoreshwa nk'igice cy'ingenzi muri sisitemu yo gufata ibinure kugira ngo ihamye pH y'ibipimo bya biyoloji n'ibikorwa byabyo.

Porogaramu:

Biology ya Molecular: Tris ikoreshwa cyane nk'ikintu gifasha mu buvuzi mu buryo butandukanye bwa biology ya molecular, harimo ADN na RNA isolation, PCR, gel electrophoresis, no gutunganya poroteyine. Ifasha mu kubungabunga ibidukikije bya pH bihamye, bigatuma habaho imiterere myiza yo gukora enzymes no gukorana kwa molecular.

Ugukura kw'uturemangingo: Tris ikunze gukoreshwa mu buryo bwo gukurura uturemangingo kugira ngo igumane pH ihoraho na osmotic ingana, ishyigikira gukura neza kw'uturemangingo no kubaho neza.

Ubutabire bwa Poroteyine: Tris ikoreshwa mu bushakashatsi bwa poroteyine, nko gushonga kwa poroteyine, gupima ubuziranenge bwa poroteyine, no kwiga uburyo poroteyine ifatana. Ifasha kugumana pH ikenewe, bigatuma poroteyine ikora neza kandi igafatana neza.

Ubumenyi bw'uturemangingo: Tris ikoreshwa mu bipimo bitandukanye bya enzyme kugira ngo yongere imiterere ya pH ikenewe kugira ngo enzyme ikore neza. Itanga ibisubizo byizewe kandi bishobora kongera kuboneka, bigatuma habaho gupima neza kinetics ya enzymes no kwiga kubuza.

Isuzuma rya Biochemical: Tris ikoreshwa nk'igice mu isuzuma ry'ibinyabuzima byinshi bitewe n'imiterere yayo yo gufunga. Igumana pH idahinduka mu isuzuma rya colorimetric, spectrophotometric, na enzyme, yongera ukuri n'ubwizerwe bw'ibisubizo.