3-HYDROXY-4-(5-NITROPYRIDYLAZO)PROPYLANI CAS:143205-66-7

Ni icyuma gipima urumuri kirimo itsinda rya nitrobenzoxadiazole (NBD), rifite ubushobozi bukomeye bwo kugaragaza urumuri. Itsinda rya aldehyde ryemerera gushyira no kumenya ibirango byihariye bya biomolecule nka poroteyine, aside nucleic, na lipide.

NBD-aldehyde ikunze gukoreshwa nk'irangi rikoreshwa mu gusuzuma no gupima uburyo poroteyine cyangwa peptide zihurirana, guhindura poroteyine, no gukorana kwa poroteyine na poroteyine. Ishobora gushyira amazina ku matsinda ya amino yigenga kuri poroteyine cyangwa peptides, bigatuma habaho imigozi ihamye ya covalent. Ibi bituma igira akamaro mu gukoresha nka immunocytochemistry, immunohistochemistry, na western blotting.

Umucyo wa NBD-aldehyde ushobora gukururwa n'urumuri rwa ultraviolet cyangwa ubururu, kandi ugatanga urumuri rw'icyatsi kibisi. Iyi miterere ituma habaho kureba no gupima ibinyabutabire byanditseho amazina hakoreshejwe uburyo bwa fluorescence microscopy cyangwa spectroscopy.