P-NITROPHENYL BETA-D-LACTOPYRANOSIDE URUBANZA: 4419-94-7

P.PNPG ni insimburangingo ishobora gukoreshwa na beta-galactosidase, bikavamo kurekura ibicuruzwa bifite ibara ry'umuhondo.Ingano ya hydrolysis ya substrate irashobora kugereranywa na spropropotometometricique mugupima iyinjizwa ryibicuruzwa ku burebure bwihariye.Ibi bifasha abashakashatsi gusuzuma ibikorwa na kinetika ya beta-galactosidase mubice bitandukanye, nko kwiga imikorere ya enzyme, gusuzuma inzitizi ya enzyme cyangwa abayikora, cyangwa gusuzuma ingaruka ziterwa na mutation kubikorwa bya enzyme.

Tegeka cyangwa usabe amagambo

Ibicuruzwa birambuye

Ibicuruzwa

Gushyira mu bikorwa n'ingaruka

Kumenya ibikorwa bya beta-galactosidase: PNPG ikoreshwa muburyo bwo gupima ibikorwa bya beta-galactosidase, enzyme itera hydrolysis ya lactose muri glucose na galaktose.Hydrolysis ya PNPG na beta-galactosidase irekura molekile ya p-nitropenol (pNP), ishobora kugaragara kuri spropropotometometricique kubera ibara ry'umuhondo.

Kugenzura inzitizi ya enzyme hamwe nabayikora: PNPG irashobora gukoreshwa mugusuzuma kwinshi-kwinjiza kugirango umenye ibice bigenga ibikorwa bya beta-galactosidase.Mugupima igipimo cya hydrolysis ya PNPG imbere yikigereranyo gitandukanye, abashakashatsi barashobora kumenya inhibitor zigabanya ibikorwa bya enzyme cyangwa abayikora bongera ibikorwa bya enzyme.

Kwiga enzyme kinetics: hydrolysis ya PNPG na beta-galactosidase ikurikira kinetics ya Michaelis-Menten, ituma abashakashatsi bamenya ibipimo byingenzi bya enzyme nkumuvuduko mwinshi wa reaction (Vmax) na Michaelis uhoraho (Km).Aya makuru afasha mugusobanukirwa enzyme ya substrate ifitanye isano na catalitiki ikora neza.

Porogaramu ya biologiya ikoreshwa: Beta-galactosidase, ifata PNPG, ikunze gukoreshwa nka gene yumunyamakuru muri biologiya ya molekuline.PNPG substrate ikoreshwa kenshi mugushakisha no kwiyumvisha imvugo ya gene yumunyamakuru, itanga uburyo bworoshye kandi bworoshye bwo gusuzuma imvugo ya gene muburyo butandukanye bwubushakashatsi.

Icyitegererezo cyibicuruzwa

Gupakira ibicuruzwa:

Amakuru yinyongera:

Ibigize	C18H25NO13
Suzuma	99%
Kugaragara	Ifu yera
URUBANZA No.	4419-94-7
Gupakira	Ntoya kandi nini
Ubuzima bwa Shelf	Imyaka 2
Ububiko	Ubike ahantu hakonje kandi humye
Icyemezo	ISO.