P-NITROPHENYL BETA-D-LACTOPYRANOSIDE CAS:4419-94-7

Kumenya imikorere ya beta-galactosidase: PNPG ikunze gukoreshwa mu isuzuma kugira ngo hamenyekane imikorere ya beta-galactosidase, enzyme ihindura hydrolysis ya lactose ikayihinduramo glucose na galactose. Hydrolysis ya PNPG na beta-galactosidase isohora molekile ya p-nitrophenol (pNP), ishobora kumenyekana mu buryo bwa spectrophotometric bitewe n'ibara ryayo ry'umuhondo.

Gusuzuma imisemburo ibuza enzyme n'ibiyitera imbaraga: PNPG ishobora gukoreshwa mu gusuzuma ibintu byinshi kugira ngo hamenyekane ibintu bihindura imikorere ya beta-galactosidase. Mu gupima igipimo cya hydrolysis ya PNPG imbere y'ibiyitera imbaraga bitandukanye, abashakashatsi bashobora kumenya ibiyitera imbaraga bigabanya imikorere ya enzyme cyangwa ibiyitera imbaraga byongera imikorere ya enzyme.

Inyigo ku mikorere ya enzyme: Uburyo PNPG ikora hidrolise hifashishijwe beta-galactosidase bukurikiza imikorere ya Michaelis-Menten, butuma abashakashatsi bashobora kumenya ibipimo by'ingenzi bya enzyme nka velocity ntarengwa ya reaction (Vmax) na Michaelis constant (Km). Aya makuru afasha mu gusobanukirwa uburyo enzyme ikora hamwe n'imikorere myiza ya catalytic.

Uburyo bwo gukoresha ibinyabuzima bya molekile: Beta-galactosidase, igabanya PNPG, ikunze gukoreshwa nk'umunyamakuru muri biology ya molekile. Igice cya PNPG gikunze gukoreshwa mu kumenya no kwiyumvisha imiterere ya gene ya reporter, bigatanga uburyo bworoshye kandi bwihuse bwo gusuzuma imiterere ya gene mu buryo butandukanye bw'igerageza.