DDT CAS: 3483-12-3 Igiciro cyabakora

DL-Dithiothreitol, izwi kandi ku izina rya DTT, ni ikintu kigabanya cyane gikoreshwa mu bushakashatsi bw’ibinyabuzima na biologiya.Ni molekile ntoya ifite itsinda rya thiol (irimo sulfure) kuri buri mpera.

DTT ikoreshwa kenshi kugirango isibe disulfide ihuza poroteyine, ifasha guhishura cyangwa kuyitandukanya.Uku kugabanya imiyoboro ya disulfide ni ngombwa muburyo butandukanye bwa laboratoire nko kweza poroteyine, gel electrophorei, hamwe nubushakashatsi bwa protein.DTT irashobora kandi gukoreshwa mukurinda amatsinda ya thiol no kwirinda okiside mugihe cyibigeragezo.

DTT isanzwe yongerwaho ibisubizo byubushakashatsi mubitekerezo bito, kandi ibikorwa byayo biterwa no kuba ogisijeni ihari.Ni ngombwa gufata neza DTT kuko yunvikana ikirere, ubushyuhe, nubushuhe, bishobora kugabanya imikorere yabyo.

Tegeka cyangwa usabe amagambo

Ibicuruzwa birambuye

Ibicuruzwa

Gushyira mu bikorwa n'ingaruka

Kugabanya ibicuruzwa bya Disulfide: DTT ikoreshwa cyane cyane mu guca imiyoboro ya disulfide, iyo ikaba ari isano ya covalent ikorwa hagati y’ibisigisigi bibiri bya sisitemu muri poroteyine.Mugabanye ubwo bucuti, DTT ifasha gutandukanya poroteyine, bigafasha kwiga imiterere n'imikorere yabyo.

Ububiko bwa poroteyine: DTT irashobora gufasha muburyo bukwiye bwa poroteyine mukurinda gushinga nabi disulfide.Igabanya imiyoboro yose itari kavukire ya disulfide ishobora gukora mugihe cyo gufunga poroteyine, bigatuma poroteyine ishobora guhinduka.

Igikorwa cya Enzyme: DTT irashobora gukora enzymes zimwe na zimwe mugabanya inzitizi zose zibuza disulfide zihari.Byongeye kandi, DTT irashobora gukumira okiside yibisigisigi bya sisitemu ikomeye, bishobora kuba nkenerwa mubikorwa bya enzyme.

Umusaruro wa Antibody: DTT isanzwe yongerwaho kugirango igabanye imiyoboro ya disulfide mugihe cyo gukora antibodies.Ifasha mukurinda gushiraho imiyoboro idahwitse ya disulfide, ishobora kubuza antigen guhuza neza.

Guhindura poroteyine: DTT irashobora gukoreshwa muguhindura poroteyine mukurinda okiside cyangwa kwegeranya.Ifasha kugumana imiterere ya poroteyine mugihe cyo kubika no kugerageza.

Kugabanya abakozi muri Biologiya ya Molecular: DTT ikoreshwa muburyo butandukanye bwa biologiya ya biologiya nka ADN ikurikirana, PCR, hamwe no kweza poroteyine.Irashobora gufasha kugumana imiterere yagabanijwe yibice bikomeye, itanga ibisubizo byiza byubushakashatsi.

Icyitegererezo cyibicuruzwa

Gupakira ibicuruzwa:

Amakuru yinyongera:

Ibigize	C4H10O2S2
Suzuma	99%
Kugaragara	Ifu yera
URUBANZA No.	3483-12-3
Gupakira	Ntoya kandi nini
Ubuzima bwa Shelf	Imyaka 2
Ububiko	Ubike ahantu hakonje kandi humye
Icyemezo	ISO.