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X-GAL CAS: 7240-90-6 Igiciro cyabakora

5-Bromo-4-chloro-3-indolyl-β-D-galactoside (X-Gal) ni insimburangingo ya chromogeneque ikoreshwa mu binyabuzima bya molekuline no mu binyabuzima.Ikoreshwa cyane mugutahura gene ya lacZ, igizwe na enzyme β-galactosidase.


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Gushyira mu bikorwa n'ingaruka

Guhindura amabara: X-Gal mubusanzwe idafite ibara ariko, kuri hydrolysis na β-galactosidase, ihinduka ubururu.Ihinduka ryamabara ryemerera gutahura no kugereranya ibikorwa bya galactosidase.

Kumenya Gene LacZ: X-Gal ikoreshwa mugutahura ingirabuzimafatizo cyangwa imiterere ya geneti igaragaza gene ya lacZ.LacZ isanzwe ikoreshwa nkumunyamakuru gene muri biologiya ya biologiya kugirango isuzume imvugo ya gene cyangwa yige ibikorwa byabateza imbere.

Kugenzura Abakoloni: X-Gal ikoreshwa kenshi mugupima kwa bagiteri.LacZ-yerekana bagiteri ya bagiteri igaragara nkubururu iyo ikuze kuri agar irimo X-Gal, ituma kumenyekana byoroshye no guhitamo coloni nziza.

Isesengura rya Gene Fusion: X-Gal nayo ikoreshwa mubushakashatsi bwa gene fusion.Iyo gene igenewe ihujwe na gen ya lacZ, irangi rya X-Gal rishobora kwerekana imiterere ya proteine ​​ya fusion muri selile cyangwa tissue.

Guhindura poroteyine: X-Gal irangi irashobora gukoreshwa mugukora iperereza rya poroteyine.Muguhuza poroteyine yinyungu kuri gene ya lacZ, ibikorwa β-galactosidase irashobora kwerekana aho poroteyine iba muri selile.

Ibigereranyo bya X-Gal: Imiterere yahinduwe ya X-Gal, nka Bluo-Gal cyangwa Red-Gal, yakozwe kugirango yemere ubundi buryo bwo guteza imbere amabara.Ibigereranyo bifasha gutandukanya lacZ-nziza na lacZ-selile selile cyangwa tissue ukoresheje amabara atandukanye.

Icyitegererezo cyibicuruzwa

7240-90-6-1
7240-90-6-2

Gupakira ibicuruzwa:

6892-68-8-3

Amakuru yinyongera:

Ibigize C14H15BrClNO6
Suzuma 99%
Kugaragara Ifu yera
URUBANZA No. 7240-90-6
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Ubuzima bwa Shelf Imyaka 2
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