X-GAL CAS: 7240-90-6 Igiciro cy'umukozi

Ihinduka ry'ibara: X-Gal ubusanzwe nta bara igira ariko, iyo ikozwe hidroliside na β-galactosidase, ihinduka ubururu. Iri hinduka ry'ibara rituma umuntu abona neza kandi agapima imikorere ya β-galactosidase.

Gupima Gene ya LacZ: X-Gal ikoreshwa mu kumenya uturemangingo cyangwa imiterere ya gene igaragaza gene ya lacZ. LacZ ikoreshwa cyane nk'igene y'umunyamakuru muri biology ya molekile kugira ngo isuzume imikorere ya gene cyangwa inyigo y'ibikorwa bya promoter.

Gupima Amatungo: X-Gal ikunze gukoreshwa mu gusuzuma udukoko twa bagiteri. Amatungo ya bagiteri agaragara muri LacZ asa n'ubururu iyo akuriwe kuri agar irimo X-Gal, bigatuma byoroha kumenya no gutoranya amatungo ya lacZ.

Isesengura rya Gene Fusion: X-Gal nayo ikoreshwa mu bushakashatsi bwo guhuza gene. Iyo gene ihuriweho na lacZ gene, X-Gal staining ishobora kugaragaza imiterere ya poroteyine yo guhuza gene mu karemangingo cyangwa mu ngingo.

Gutandukanya poroteyine: Gutandukanya X-Gal bishobora gukoreshwa mu gusuzuma aho poroteyine iri mu turemangingo. Mu guhuza poroteyine ishimishije na gene ya lacZ, imikorere ya β-galactosidase ishobora kwerekana aho poroteyine iherereye mu turemangingo.

X-Gal Analogues: Imiterere ya X-Gal yahinduwe, nka Bluo-Gal cyangwa Red-Gal, yakozwe kugira ngo yemererwe ubundi buryo bwo guteza imbere amabara. Izi analogues zituma habaho gutandukanya uturemangingo cyangwa ingirabuzimafatizo za lacZ-positive na lacZ-negative hakoreshejwe amabara atandukanye.