HEPES CAS: 7365-45-9 Igiciro cy'umukozi

HEPES (4- (2-Hydroxyethyl)piperazine-1-ethanesulfonic aside) ni igikoresho cy’ibinyabuzima gikunze gukoreshwa mu bushakashatsi ku binyabuzima no mu bya shimi. Kizwiho ubushobozi bwo kugumana pH ihamye mu bisubizo by’amazi, bigatuma kiba cyiza mu bikorwa bitandukanye birimo inzira z’uturemangingo n’iz’uturemangingo. HEPES ni imvange ya zwitterionic, bivuze ko ifite imbaraga nziza n’imbi ku matsinda atandukanye y’imikorere, ibi bikaba bituma ishobora kurwanya impinduka muri pH ziterwa no kongeramo aside cyangwa base. Ikunze gukoreshwa mu miterere y’uturemangingo, isuzuma rya enzymes, inyigo za poroteyine, igerageza rya electrophoresis, n’imiti.

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Ishyirwa mu bikorwa n'ingaruka

Kugabanya pH: HEPES ikoreshwa cyane nk'igikoresho cyo kugabanya pH kugira ngo igumane pH ihamye mu mikorere y'uturemangingo no mu bipimo by'ibinyabuzima. Ikora neza mu rugero rwa pH y'umubiri kuva kuri 6.8 kugeza 8.2, bigatuma iba nziza mu kubungabunga imiterere myiza y'imikorere y'ibinyabuzima.

Ubuvuzi bw'uturemangingo: HEPES ikunze kongerwa mu buryo bwo gukwirakwiza pH mu turemangingo kugira ngo ihamye urugero rwa pH, bitanga ahantu hakwiye ho gukura kw'uturemangingo no gukomeza kubaho neza. Bifasha gukumira ihindagurika rya pH rishobora kugira ingaruka mbi ku myitwarire y'uturemangingo n'ibisubizo by'igerageza.

Isuzuma rya enzyme: HEPES ikunze gukoreshwa nk'uburyo bwo kwirinda ingaruka za enzyme bitewe n'ubushobozi bwayo bwo kugumana pH runaka. Ifasha mu kubungabunga imikorere myiza ya enzyme no kudahungabana, bityo yongera ubwiza n'ubudahemuka bw'isuzuma rya enzyme.

Inyigo za poroteyine: HEPES ikoreshwa mu bushakashatsi butandukanye bujyanye na poroteyine, harimo gutunganya poroteyine, gushushanya poroteyine, no gusesengura imiterere ya poroteyine. Ifasha mu gutuma pH ihinduka no kugumana ipfundo ryiza rya poroteyine no kugumana ipfundo rihamye muri ubwo bushakashatsi.

Electrophoresis: HEPES ikoresha uburyo bwa gel electrophoresis, nka SDS-PAGE na agarose gel electrophoresis. Ikoreshwa nk'ikintu gikingira mu gice gikoresha amashanyarazi, igatanga imiterere ihamye ya pH yo gutandukanya no gusesengura biomolecules.

Imiti: HEPES ikoreshwa mu gukora imiti n'ikoranabuhanga. Ifasha mu kubungabunga ubusugire n'ubuziranenge bw'ibintu bikora, bigatuma bikora neza kandi bikamara igihe kirekire.

Gupakira ibicuruzwa:

Amakuru y'inyongera:

Imiterere	C8H18N2O4S
Isuzuma	99%
Isura	Ifu y'umweru
Nimero ya CAS	7365-45-9
Gupakira	Bito kandi byinshi
Igihe cyo kuruhuka	Imyaka 2
Ububiko	Bika ahantu hakonje kandi humutse
Icyemezo	ISO.