Uridine,2′-deoxy-5-ethynyl- CAS:61135-33-9

5-ethynyl-2′-deoxyuridine (EdU) ni analoge ya nucleoside ikoreshwa cyane mu bijyanye n'ubushakashatsi ku binyabuzima bya molekile n'ibinyabuzima by'uturemangingo. Ikunze gukoreshwa mu gushyira ibimenyetso no kumenya ADN nshya yakozwe mu turemangingo tuzima binyuze mu buryo buzwi nka "click chemistry". Ubu buryo butuma umuntu abona neza kandi agapima uko ADN yiyongera ndetse no kwiyongera kw'uturemangingo. EdU yabaye ubundi buryo buzwi bwo gusimbura thymidine analog bromodeoxyuridine (BrdU) bitewe nuko ihura na mikorosikopi ya fluorescence ndetse no kutagira intambwe zikomeye zo guhindura ADN mu gihe cyo kuyimenya. Byongeye kandi, imiterere ya chimique ya EdU ituma habaho uburyo bwihariye bwo kuyimenya, bigatuma iba igikoresho cy'agaciro mu kwiga imikorere ya ADN n'imikorere y'uturemangingo.

Gutumiza cyangwa Gusaba Igiciro

Ibisobanuro birambuye ku gicuruzwa

Ibirango by'ibicuruzwa

Ishyirwa mu bikorwa n'ingaruka

Isuzuma ry’ubwiyongere bw’uturemangingo: EdU ishobora gushyirwa mu gukora ADN mu cyiciro cya S cy’ingengabihe y’uturemangingo. Ibi bituma iba igikoresho cy’ingirakamaro mu kwiga ikwirakwira ry’uturemangingo no gukora ADN.

Ubuhanga bwa Click Chemistry: EdU ikunze gukoreshwa hamwe na cliqueur ya click, uburyo bwo gutoranya kandi butanga umusaruro mwinshi bukoreshwa mu gupima biomolecules. Ubu buryo butuma haboneka ADN ya EdU mu turemangingo no mu turemangingo.

Mikorosikopi ya Fluorescence: Gushyiramo EdU bituma habaho ishusho y’imikorere ya ADN hifashishijwe mikorosikopi ya fluorescence. Ubu buryo butanga igikoresho cy'ingirakamaro mu kwiga igabanyuka ry'uturemangingo no kwiyongera kwatwo.

Gupakira ibicuruzwa:

Amakuru y'inyongera:

Imiterere	C11H12N2O5
Isuzuma	99%
Isura	Ifu y'umweru
Nimero ya CAS	61135-33-9
Gupakira	Bito kandi byinshi
Igihe cyo Kubika	Imyaka 2
Ububiko	Bika ahantu hakonje kandi humutse
Icyemezo	ISO.