URUBUGA RWA ONPG: 369-07-3 Igiciro cyabakora

Ingaruka ya ONPG nka substrate igomba guhindurwa na enzyme β-galactosidase, bikavamo kurekura ibicuruzwa byumuhondo, o-nitropenol.Ihinduka ryibara rishobora gupimwa spekitifotometometrike, ryemerera kugereranya ibikorwa β-galactosidase.Ikoreshwa rya ONPG cyane cyane mugusuzuma imvugo ya gene mubinyabuzima bwa molekuline nubushakashatsi bwa mikorobi.Bikunze gukoreshwa mugupima β-galactosidase nkumunyamakuru wubushakashatsi bwerekana imiterere ya gene, cyane cyane muri bagiteri nka E. coli.Gene ya lacZ, ikubiyemo β-galactosidase, ikoreshwa kenshi nkikimenyetso cyo gusesengura imvugo ya gene, kuko imvugo yayo ishobora guterwa nuburyo bwihariye cyangwa igenzurwa nabateza imbere. Isuzuma rya ONPG ritanga uburyo bworoshye kandi bwizewe bwo gusuzuma urwego rwa imvugo ya gene mugupima ibikorwa bya gal-galactosidase.Ubu bushakashatsi bukoreshwa cyane mubikorwa bitandukanye, nko kwiga ibikorwa bya porotokoro, kugenzura gene, hamwe na poroteyine-proteyine.Byongeye kandi, irashobora gukoreshwa muguhitamo enzyme kinetics no gusuzuma ingaruka ziterwa na mutation cyangwa imiti kubikorwa bya enzyme.