HEPES-Na CAS: 75277-39-3 Igiciro cyabakora

Buffering agent: HEPES umunyu wa sodium ukunze gukoreshwa nkibikoresho byoherejwe mubushakashatsi bwibinyabuzima na biohimiki.Ifasha kugumana urwego ruhamye rwa pH, cyane cyane murwego rwa physiologique (pH 7.2-7.6).Ubushobozi bwayo bwogukora butanga agaciro mugukomeza imiterere ikwiye yimikorere itandukanye, umuco wimikorere, hamwe nubuhanga bwibinyabuzima bwa molekile.

Umuco w'akagari: HEPES umunyu wa sodiumi ukoreshwa cyane nkumukozi woherejwe mubitangazamakuru byumuco.Ubushobozi bwo gukomeza pH ihamye ningirakamaro kugirango imikurire ikwiye kandi ibe ingirabuzimafatizo.HEPES ikunze gukundwa kurenza izindi mikoreshereze kuko itagaragaza impinduka zikomeye muri pH iyo ihuye na CO2 yo mu kirere.

Enzyme yiga: HEPES umunyu wa sodiumi ni ingirakamaro cyane mubushakashatsi bwimisemburo aho bikenewe pH ihoraho kandi igenzurwa.Irinda ihindagurika rikomeye muri pH mugihe cya reaction enzymatique, ikemeza ibikorwa byiza bya enzyme.

Electrophoresis: HEPES umunyu wa sodium ukunze gukoreshwa nkumuti wa tekinike mu buhanga butandukanye bwa electrophoreque nka polyacrylamide gel electrophorei (PAGE) na agarose gel electrophorei.Ifasha kubungabunga pH ya buffer, ningirakamaro mu gutandukanya no gusesengura acide nucleic na proteyine.

Ubushakashatsi bwibinyabuzima: HEPES umunyu wa sodium ukunze gukoreshwa muburyo butandukanye bwibinyabuzima, harimo imisemburo ya enzyme, ibipimo bya poroteyine, hamwe na spropropotometometric.Ifasha kugumana icyifuzo cya pH gikenewe kubisubizo nyabyo kandi byororoka.

Gutegura ibiyobyabwenge: HEPES umunyu wa sodiumi ukoreshwa no mumiti ya farumasi nkibikoresho byo guhagarika imiti no gukomeza pH yifuza.