FLUORESCEIN MONO-BETA-D- URUBANZA RWA GALACTOPYRANOSIDE: 102286-67-9

Fluorescein mono-beta-D-galactopyranoside, izwi kandi ku izina rya FMG, ni uruganda rwa fluorescent rukunze gukoreshwa nka substrate mu bushakashatsi butandukanye bwibinyabuzima na selile.Bikomoka kuri methyl-beta-D-galactopyranoside mu kuyihuza na molekile ya fluorescein.FMG ikoreshwa cyane mu kwiga ibikorwa bya beta-galactosidase, enzyme itera hydrolysis ya lactose muri galaktose na glucose.Ukoresheje FMG nka substrate, abashakashatsi barashobora gukurikirana ibikorwa byimikorere ya beta-galactosidase binyuze mugupima imyuka ya fluorescence.Hydrolysis ya FMG na beta-galactosidase itera kurekura fluorescein, bigatuma habaho kwiyongera kwa signal ya fluorescent ishobora kugereranywa.Iyi nteruro nayo ikoreshwa mugukora iperereza kumyuka ya karubone.FMG irashobora gukoreshwa nka probe ya molekulari kugirango yige isano ihuza inyigisho (proteyine zihuza cyane na karubone) na galaktose irimo karubone.Guhuza ibigo bya FMG-bigisha bishobora gutahurwa no kugereranywa hashingiwe ku mpinduka zatewe na florescence. Muri rusange, FMG ni igikoresho kinini mu kwiga ibikorwa bya enzyme no kumenyekanisha karubone, bitanga uburyo bworoshye kandi bworoshye bwo gupima fluorescence no gusuzuma ibyo binyabuzima.

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Fluorescein mono-beta-D-galactopyranoside (FMG) ni molekile ikunze gukoreshwa mubushakashatsi bwibinyabuzima nka substrate kugirango hamenyekane ahari enzyme ya beta-galactosidase.FMG ni inkomoko ya sukari lactose kandi ihujwe na molekile ya fluorescein.

Ingaruka nyamukuru ya FMG nuko iba hydrolyzed na beta-galactosidase, enzyme igabanya lactose muri galaktose na glucose.Iyi hydrolysis ya enzymatique ya FMG itera kurekura fluorescein, itanga ikimenyetso gikomeye cya fluorescence.

Porogaramu yibanze ya FMG ni mukumenya no gupima ibikorwa bya beta-galactosidase mubitegererezo bitandukanye.Iyi misemburo iboneka mu binyabuzima byinshi, harimo na bagiteri na selile z’inyamabere, kandi ibikorwa byayo birashobora kwerekana inzira zitandukanye za selile n'inzira za metabolike.

Ukoresheje FMG nka substrate, ibikorwa bya beta-galactosidase birashobora gupimwa mugukurikirana fluorescence itangwa na fluorescein yabohowe.Iki gipimo gishobora gukorwa muburyo butandukanye bwubushakashatsi, harimo muri vitro assays hamwe nubushakashatsi bwerekana amashusho ya selile.

Byongeye kandi, FMG irashobora gukoreshwa nkigikoresho cyo kwiga ikwirakwizwa no gukwirakwiza beta-galactosidase muri selile.Bakoresheje tekinoroji ya microscopi ya fluorescent, abashakashatsi barashobora kwiyumvisha fluorescence itangwa na FMG kuri hydrolysis, ibemerera gukurikirana ibikorwa byumwanya nigihe gito cya beta-galactosidase.

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Amakuru yinyongera:

Ibigize	C26H22O10
Suzuma	99%
Kugaragara	Ifu yera
URUBANZA No.	102286-67-9
Gupakira	Ntoya kandi nini
Ubuzima bwa Shelf	Imyaka 2
Ububiko	Ubike ahantu hakonje kandi humye
Icyemezo	ISO.