FLUORESCEIN MONO-BETA-D- GALACTOPYRANOSIDE CAS:102286-67-9

Fluorescein mono-beta-D-galactopyranoside, izwi kandi nka FMG, ni igikoresho gitanga urumuri gikunze gukoreshwa nk'igice cy'ingenzi mu bushakashatsi butandukanye bwa biochemical na biology y'uturemangingo. Gikomoka kuri methyl-beta-D-galactopyranoside mu kuyihuza na molecule ya fluorescein.FMG ikoreshwa cyane mu kwiga imikorere ya beta-galactosidase, enzyme ihindura lactose muri galactose na glucose. Mu gukoresha FMG nk'igice cy'ingenzi, abashakashatsi bashobora gukurikirana imikorere ya enzyme ya beta-galactosidase binyuze mu gupima imyuka ya fluorescence. Hydrolysis ya FMG na beta-galactosidase ituma fluorescein irekurwa, bigatuma ikimenyetso cya fluorescent cyiyongera gishobora gupimwa. Iki gice kandi gikoreshwa mu gusuzuma uburyo karubohidrati imenyekana n'imikoranire yayo. FMG ishobora gukoreshwa nk'igikoresho cy'ubushakashatsi mu kwiga uburyo lectins (poroteyine zifatana by'umwihariko na karubohidrati) zifatana na karubohidrati irimo galactose. Uburyo bwo gufatanya kwa FMG-lectin complexes bushobora kumenyekana no gupimwa hashingiwe ku mpinduka mu isohoka ry'urumuri. Muri rusange, FMG ni igikoresho gifite uburyo bwinshi bwo kwiga imikorere ya enzymes no kumenya karubohidrati, gitanga uburyo bworoshye kandi bwihuse bwo gupima urumuri no gusuzuma iyi mikorere y'ibinyabuzima.

Gutumiza cyangwa Gusaba Igiciro

Ibisobanuro birambuye ku gicuruzwa

Ibirango by'ibicuruzwa

Ishyirwa mu bikorwa n'ingaruka

Fluorescein mono-beta-D-galactopyranoside (FMG) ni molekile ikunze gukoreshwa mu bushakashatsi bw’ibinyabuzima nk'ikintu gifasha kumenya niba enzyme ya beta-galactosidase ihari kandi ikora. FMG ni ikintu gikomoka kuri lactose y’isukari kandi gihuzwa na molekile ya fluorescein.

Ingaruka nyamukuru ya FMG ni uko ikoreshwa cyane cyane na beta-galactosidase, enzyme isenya lactose ikayihinduramo galactose na glucose. Iyi enzyme hydrolysis ya FMG ituma fluorescein irekurwa, itanga ikimenyetso gikomeye cy’urumuri.

Uburyo bw'ibanze bwo gukoresha FMG ni ukumenya no gupima imikorere ya beta-galactosidase mu bipimo bitandukanye. Iyi enzyme iboneka mu binyabuzima byinshi, harimo bagiteri n'uturemangingo tw'inyamabere, kandi imikorere yayo ishobora kuba ikimenyetso cy'imikorere itandukanye y'uturemangingo n'inzira z'imikorere y'uturemangingo.

Mu gukoresha FMG nk'igice cy'umubiri, ibikorwa bya beta-galactosidase bishobora gupimwa hagenzurwa fluorescence itangwa na fluorescein yakuwemo. Iki gipimo gishobora gukorwa mu buryo butandukanye bwo kugerageza, harimo ibizamini byakozwe muri vitro n'inyigo zo gufata amashusho y'uturemangingo tuzima.

Byongeye kandi, FMG ishobora gukoreshwa nk'igikoresho cyo kwiga uko beta-galactosidase ikwirakwira n'aho iherereye mu turemangingo. Bakoresheje tekiniki za mikorosikopi y'urumuri, abashakashatsi bashobora kubona urumuri rutangwa na FMG mu gihe cyo gushonga, bigatuma bakurikirana imikorere ya beta-galactosidase mu mwanya n'igihe.

Urugero rw'ibicuruzwa

Gupakira ibicuruzwa:

Amakuru y'inyongera:

Imiterere	C26H22O10
Isuzuma	99%
Isura	Ifu y'umweru
Nimero ya CAS	102286-67-9
Gupakira	Bito kandi byinshi
Igihe cyo kuruhuka	Imyaka 2
Ububiko	Bika ahantu hakonje kandi humutse
Icyemezo	ISO.