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Heppso sodium CAS: 89648-37-3 Igiciro cy'uruganda
Umunyu wa sodium N-[2-Hydroxyethyl]piperazine-N'-[2-hydroxypropanesulfonic aside] ni umunyu w’ibinyabutabire ufite formula ya C8H19N2NaO4S. Ni umunyu wa sodium ukomoka kuri piperazine, urimo hydroxyethyl na aside hydroxypropanesulfonic. Ukoreshwa cyane mu nganda zikora imiti nk'umuti ugabanya ubukana n'umuti utera imbaraga mu mikorere y'imiti. Iki kinyabutabire gifasha kubungabunga pH n'umuti uhoraho.
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P-NITROPHENYL BETA-D-LACTOPYRANOSIDE CAS:4419-94-7
P-Nitrophenyl beta-D-lactopyranoside, izwi kandi nka PNPG, ni ikintu gikunze gukoreshwa mu isuzuma rya enzymatic kugira ngo hamenyekane imikorere ya beta-galactosidase, enzyme igira uruhare mu mikorere ya karubohidrati. PNPG ni substrate y’ubukorano ishobora gucibwamo na beta-galactosidase, bigatuma habaho irekurwa ry’umuhondo. Ingano ya substrate hydrolysis ishobora gupimwa hifashishijwe spectrophotometrical hapimwa uburyo iki kinyabutabire gishyirwa ku rugero runaka. Ibi bituma abashakashatsi bashobora gusuzuma imikorere n’uburyo beta-galactosidase ikora mu buryo butandukanye, nko kwiga imikorere ya enzyme, gusuzuma uburyo enzyme ibuza cyangwa ikora, cyangwa gusuzuma ingaruka z’impinduka ku mikorere ya enzyme.
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popso sesquisodium CAS: 108321-08-0
Umunyu wa sesquisodium wa Piperazine-N,N'-bis (aside 2-hydroxypropanesulfonic), uzwi kandi ku izina rya PIPES sesquisodium salt, ni ikinyabutabire gikoreshwa nk'ikintu gikingira mu bintu bitandukanye bya siyansi n'inganda. Gifasha mu kubungabunga urwego rwa pH ruhamye mu bisubizo, cyane cyane mu rugero rwa pH y'umubiri. Umunyu wa sesquisodium wa PIPES ukunze gukoreshwa mu buryo bw'imiti, mu binyabuzima bya poroteyine, mu buryo bwa electrophoresis, mu buhanga bwa molekile, no mu buryo bwo gutanga imiti. Ukora nk'umugenzuzi wa pH no kongera imikorere ya enzyme no guhagarara, bigatuma ugira agaciro mu bushakashatsi bwinshi n'imikorere y'inganda.
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ACES CAS: 7365-82-4 Igiciro cy'umukozi
Aside N-(2-Acetamido)-2-aminoethanesulfonic, izwi kandi nka ACES, ni umuti ukoreshwa cyane mu bushakashatsi bwa siyansi. Ifasha mu kubungabunga pH ihamye mu bisubizo kandi irashongesha cyane mu mazi. ACES ifite uburozi buke kandi ntigira ingaruka nyinshi ku mikorere ya biochemical, bigatuma ikoreshwa mu buryo butandukanye. Ikunze gukoreshwa nk'umuti urinda poroteyine mu bushakashatsi bwo kubungabunga poroteyine no mu isuzuma rya enzymes, aho ikomeza imiterere myiza yo gukora poroteyine no gukora enzymes.
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Phenylgalactoside CAS: 2818-58-8
Phenylgalactoside, izwi kandi ku izina rya p-nitrophenyl β-D-galactopyranoside (pNPG), ni substrate y’ubukorano ikoreshwa cyane mu bushakashatsi bwa biochemical na molekile. Ikunze gukoreshwa mu gupima no gupima imikorere ya enzyme β-galactosidase.
Iyo phenylgalactoside ihiye na β-galactosidase, isohora p-nitrophenol, ikaba ari ibara ry'umuhondo. Irekurwa rya p-nitrophenol rishobora gupimwa mu buryo bw'imibare hakoreshejwe spectrophotometer, kuko ubushobozi bwa p-nitrophenol bushobora kugaragara ku bunini bwa 405 nm.
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DIPSO CAS: 68399-80-4 Igiciro cy'umukozi
DIPSO isobanura "Diisopropyl azodicarboxylate," ikaba ari reagent ikoreshwa cyane muri chimie organic. Ikoreshwa cyane cyane muri Mitsunobu reaction, ikaba ari uburyo bwo guhindura alcool mo amatsinda atandukanye y'imikorere nka esters, ethers, cyangwa amines. DIPSO ikora nk'isoko y'ikintu gifasha cyane mu gukora cyane cyitwa azodicarboxylate, ibyo bikaba byoroshya iyi mpinduka.
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4-Nitrophenyl-beta-D-glucopyranoside CAS: 2492-87-7
4-Nitrophenyl-beta-D-glucopyranoside ni substrate ikoreshwa cyane mu bushakashatsi bwa biochemical kugira ngo hamenyekane imikorere ya enzymes nka β-glucuronidase. Iyi enzyme ikoreshwa na hydrolyzed na enzyme, bigatuma 4-nitrophenol irekurwa, ishobora gupimwa hakoreshejwe spectrophotometry. Ikoreshwa ryayo rifasha abashakashatsi kwiga ibintu bitandukanye bijyanye n'imikorere y'imiti, uburozi, n'isuzuma ry'ubuvuzi rijyanye n'ingaruka za glucuronidation.
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Tris maleate CAS: 72200-76-1
Tris maleate ni ikinyabutabire gikora nk'ikinyabutabire cya pH mu nganda zitandukanye. Ikoreshwa mu kubungabunga urugero rwa pH rudahinduka no kurwanya impinduka ziterwa no kongeramo aside cyangwa base. Tris maleate ikunze gukoreshwa mu bushakashatsi ku binyabuzima, gutunganya poroteyine, mu nganda, no mu buhanga bwo gusesengura. Igira akamaro cyane cyane mu kugabanya pH ku rugero rwo hasi kandi izwiho ubuhanga bwayo mu kubungabunga imiterere myiza ya pH.
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MOBS CAS:115724-21-5 Igiciro cy'umukozi
4-morpholin-4-ylbutane-1-aside sulfonic, izwi kandi nka MOBS, ni ikintu cy’umwimerere gikoreshwa mu bushakashatsi ku binyabuzima no mu buvuzi bwa biokemike. Gihamye kandi gishobora kugumana pH ihamye mu rugero rwa alkaline rudafite aho rubogamiye.BS ikoreshwa cyane mu gupima uturemangingo, gupima enzymes, ubuhanga bwa molekile, no gukoresha electrophoresis. Irahuye n'ibintu bitandukanye kandi izwiho kudahinduka no kurwanya impinduka mu bushyuhe na pH.
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1,2,3,4,6-penta-O-acetyl-alpha-D-galactopyranose CAS:4163-59-1
1,2,3,4,6-penta-O-acetyl-alpha-D-galactopyranose ni ikinyabutabire kiri mu muryango wa karubohidrati. Ni ikomoka kuri alpha-D-galactopyranose, isukari isanzwe. Iki kinyabutabire gifite amatsinda atanu ya acetyl afatanye n'amatsinda yihariye ya hydroxyl kuri molekile y'isukari. Ikunze gukoreshwa mu bintu bitandukanye bya shimi n'imiti, harimo no gutangira gukora ibindi bintu. Imiterere yacyo ya acetyl yongera ubushobozi bwayo bwo kugumana imbaraga no gukora neza, bigatuma iba ingenzi mu buhanga bwa shimi.
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POPSO CAS: 68189-43-5 Igiciro cy'umukozi
POPSO, mu magambo ahinnye ya Piperazine-N,N'-bis (aside 2-hydroxypropanesulfonic) sesquisodium salt, ni umuti ukoreshwa cyane mu bushakashatsi ku binyabuzima no mu buvuzi bwa biochemical. Ifasha mu kubungabunga urwego rwa pH ruhamye mu bisubizo, cyane cyane mu rugero rwa pH y'umubiri. Umunyu wa sesquisodium wa PIPES ukoreshwa mu mikurire y'uturemangingo, poroteyine biochimie, electrophoresis, tekiniki za biology ya molekile, uburyo bwo gutanga imiti, n'ibindi. Ubushobozi bwayo bwo kugenzura pH butuma iba igikoresho cy'agaciro mu bushakashatsi butandukanye no mu nganda.
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BES CAS:10191-18-1 Igiciro cy'umukozi
Aside N,N-Bis (hydroxyethyl)-2-aminoethanesulfonic, izwi kandi nka BES cyangwa N,N-Bis (2-hydroxyethyl)aminoethanesulfonic aside, ni imvange y'ibinyabutabire ikoreshwa cyane nk'ikintu gikingira mu buryo butandukanye bwa siyansi n'inganda.
BES ni imvange ya zwitterionic, bivuze ko ifite charges nziza na negative mu miterere yayo. Iyi miterere ituma igumana pH ihamye mu bisubizo.
BES ifite agaciro ka pKa ka kangana na 7.4, bigatuma igira akamaro kanini mu kugabanya urugero rwa pH mu mubiri. Ikunze gukoreshwa mu bushakashatsi bw’ibinyabuzima n’ubw’imiti, nko gutunganya poroteyine, gukora enzymes, no guteza imbere uturemangingo, aho kubungabunga pH runaka ari ingenzi cyane.
Byongeye kandi, BES ikoreshwa cyane mu buhanga bwa electrophoresis, kuko ifasha kugumana pH ikenewe mu gutandukanya no gusesengura biomolecules zifite ingufu, nka poroteyine na aside nucleic.
