4-Nitrophenyl beta-D-galactopyranoside CAS: 200422-18-0

Ingaruka: ONPG ni substrate ikoreshwa cyane cyane kugirango hamenyekane ahari n'ibikorwa bya enzyme β-galactosidase.Iyo β-galactosidase enzyme ihari kandi ikora, ifata ONPG mubicuruzwa bibiri: o-nitropenol hamwe nibikomoka kuri galaktose.Kwibohoza o-nitropenol bivamo ibara ry'umuhondo, rishobora gupimwa ukoresheje spekitifotometero.

Gusaba: ONPG ifite porogaramu nyinshi mubinyabuzima bwa molekuline nubushakashatsi bwibinyabuzima:

Kumenyekanisha ibikorwa β-galactosidase: ONPG isanzwe ikoreshwa mugupima no kugereranya ibikorwa bya β-galactosidase enzyme.Igipimo cyo gukora o-nitropenol, gihwanye neza nigikorwa cya enzyme, gishobora gupimwa spekitifoto.

Imvugo nogutondekanya ibisekuru: ONPG ikoreshwa kenshi mubushakashatsi bujyanye no kwerekana imiterere ya gene hamwe nubushakashatsi.Ikoreshwa cyane muri poroteyine ya fusion, nka sisitemu ya lacZ ikoreshwa cyane, kugirango yige imvugo ya gen iyobowe na porotokore yihariye.Igikorwa cya beta-galactosidase yapimwe ukoresheje ONPG itanga ubushishozi murwego rwo kwerekana gene.

Kugaragaza ibikorwa β-galactosidase: ONPG irashobora gukoreshwa nkuburyo bwo gusuzuma amabara muburyo bwa tekinoroji ya ADN kugirango hamenyekane ahari imikorere ya gene ya LacZ, ikubiyemo β-galactosidase.Ubu buryo bwo gusuzuma bufasha mukumenya clone zirimo gene yinyungu.

Enzyme kinetics yiga: ONPG nayo ifite akamaro mukwiga kinetic ya β-galactosidase enzyme.Mugupima igipimo cya enzyme-substrate reaction yibitekerezo bitandukanye, birashoboka kumenya ibipimo bya kinetic nka Michaelis-Menten constants (Km) nigipimo ntarengwa (Vmax).