4-Nitrophenyl beta-D-galactopyranoside CAS:200422-18-0

Ingaruka: ONPG ni substrate ikoreshwa by'umwihariko mu kumenya niba enzyme β-galactosidase ihari kandi ikora. Iyo enzyme β-galactosidase ihari kandi ikora, igabanya ONPG mo ibintu bibiri: o-nitrophenol n'icyavuye muri galactose. Kurekurwa kwa o-nitrophenol bivamo impinduka mu ibara ry'umuhondo, bishobora gupimwa hakoreshejwe spectrophotometer.

Ikoreshwa: ONPG ifite akamaro kenshi mu bushakashatsi ku binyabuzima bya molekile na biochimie:

Kumenya imikorere ya β-galactosidase: ONPG ikoreshwa cyane mu gupima no gupima imikorere ya enzyme ya β-galactosidase. Igipimo cyo kwirema kwa o-nitrophenol, gihuye neza n'imikorere ya enzyme, gishobora gupimwa hifashishijwe spectrophotometric.

Imiterere n'imikorere ya za gene: ONPG ikunze gukoreshwa mu bushakashatsi bujyanye n'imikorere ya za gene n'inyigo z'imikorere. Ikunze gukoreshwa mu gusuzuma poroteyine zivanze, nka sisitemu isanzwe ikoreshwa ya lacZ, kugira ngo yige imiterere ya za gene igenzurwa n'ibintu byihariye biyitera inkunga. Igikorwa cya beta-galactosidase gipimwe hakoreshejwe ONPG gitanga ibisobanuro ku rwego rw'imikorere ya za gene.

Gusuzuma ibikorwa bya β-galactosidase: ONPG ishobora gukoreshwa nk'uburyo bwo gusuzuma colorimetric mu ikoranabuhanga rya ADN rihuza kugira ngo hamenyekane niba gene ya LacZ ihari kandi ikora, ikaba ikora β-galactosidase. Ubu buryo bwo gusuzuma bufasha mu kumenya clones zirimo gene ishimishije.

Inyigo ku bijyanye na enzyme kinetics: ONPG nayo ifite akamaro mu kwiga kinetics ya enzyme ya β-galactosidase. Mu gupima igipimo cya enzyme-substrate reaction ku bipimo bitandukanye bya substrate, birashoboka kumenya ibipimo bya kinetics nka Michaelis-Menten constants (Km) na maximum reaction rate (Vmax).