4-Methylumbelliferyl-beta-D-glucopyranoside CAS: 18997-57-4

Ingaruka ya 4-Methylumbelliferyl-beta-D-glucopyranoside (MUG) nugukora nka substrate ya enzyme beta-glucosidase.Iyi misemburo ikuraho glucosideque ya MUG, bigatuma irekurwa rya 4-Methylumbelliferone (4-MU) .Gukoresha MUG cyane cyane mubijyanye na mikorobe, cyane cyane mugushakisha no kumenya bagiteri zitanga beta-glucosidase.MUG ikoreshwa cyane mugutahura Escherichia coli (E. coli) mumazi no mubiribwa.E. coli ifite enzyme beta-glucosidase, ishobora hydrolyze MUG ikanatanga ibimenyetso bya fluorescent imbere yumucyo ultraviolet (UV ).Umutungo wa fluorescent wa 4-MU utuma habaho gutahura no kubara byoroshye.Iyo substrate ya MUG ifite hydrolyzed, 4-MU yakozwe isohora fluorescence yubururu, byoroha kumenya bagiteri zifite ibikorwa bya beta-glucosidase.Ubu buryo bukunze gukoreshwa mugukurikirana ibidukikije no gupima umutekano wibiribwa, kuko butanga uburyo bwihuse kandi bworoshye bwo kumenya kwandura bagiteri. Usibye kuba ikoreshwa muri mikorobi, MUG irashobora kandi gukoreshwa mukwiga ibikorwa no kubuza beta-glucosidase muri ubushakashatsi bwibinyabuzima nubushakashatsi bwibinyabuzima.Florescence yayo ituma gupima kinetics ya enzyme kandi irashobora gukoreshwa mugupima ibishobora kubuza cyangwa gukora ibikorwa bya beta-glucosidase.Muri rusange, MUG ni uruganda rwinshi rusanga ikoreshwa cyane mubijyanye na mikorobi, ibinyabuzima, na biologiya ya biologiya kugirango bimenyekane. y'ibikorwa bya beta-glucosidase no kumenya bagiteri zitanga iyi misemburo.