SYBR GREEN I Nucleic Acid Gel Ibara CAS: 163795-75-3

Electrophoresis ya gel ya ADN: Ibara rya SYBR Green I ryongerwa kuri gel ya ADN nyuma ya electrophoresis, kandi iyo ishyizwe ku rumuri rwa UV, ifatana n'imirongo ya ADN, igatanga urumuri rwinshi. Ubu buryo bwo gusiga ibara bufasha kubona no gusesengura ibice bya ADN bitandukanyijwe n'ingano.

PCR y'ingano (qPCR): SYBR Green I ikunze gukoreshwa muri qPCR mu gukurikirana izamuka rya ADN cyangwa RNA mu gihe nyacyo. Irangi rifatana na ADN ifite imigozi ibiri ikorwa mu gihe cyo guteranya PCR, kandi kwiyongera k'ubukana bwa fluorescence gupimwa kuri buri ruziga rwo guteranya, bigatuma hamenyekana urugero rwa aside nucleic itangira.

RNA gel electrophoresis: SYBR Green I irashobora kandi gukoreshwa kuri RNA gel electrophoresis, bigatuma habaho kureba no gusesengura ibice bya RNA. Ni ingirakamaro mu gusuzuma ubuziranenge bwa RNA no kwiga urwego rw'imikorere ya RNA.

Gushyira no gutahura aside nucleic: SYBR Green I ishobora gukoreshwa mu gushyira ikimenyetso cy'aside nucleic hamwe n'urumuri. Ibi bishobora kugerwaho hakoreshejwe irangi mu gihe cyo gukora aside nucleic cyangwa hakoreshejwe uburyo bwo gushyira ikimenyetso nyuma yo gushyira ikimenyetso. Aside nucleic zigaragara zishobora kugaragazwa no kumenyekana hakoreshejwe sisitemu yo gushushanya fluorescent.