Proteinase K kuri alubumu ya tritirachium CAS:39450-01-6

Proteinase K ni enzyme ivanwa muri alubumu ya Tritirachium. Ni serine protease ifite umwihariko wagutse, bivuze ko ishobora gutandukanya imigozi ya peptide muri poroteyine zitandukanye. Proteinase K izwiho ubushobozi bwo kwangiza poroteyine neza, bigatuma iba igikoresho cy'agaciro mu bikorwa bitandukanye.

Imwe mu mikoreshereze y'ingenzi ya Proteinase K ni mu bushakashatsi ku binyabuzima bya molekile. Ikunze gukoreshwa mu gukurura ADN na RNA kugira ngo ikurweho poroteyine zishobora kubangamira isesengura ry’inyuma. Proteinase K ifasha mu gusya uturemangingo no kwangiza poroteyine z’uturemangingo, bigatuma aside nucleic nziza zitandukanya.

Proteyine K ikoreshwa cyane mu gusesengura poroteyine no mu bushakashatsi ku miterere yayo. Ishobora gukoreshwa mu gusya poroteyine, bigatuma habaho ibice bya peptide bishobora gupimwa hakoreshejwe uburyo nka spectrometry. Byongeye kandi, Proteyine K ni ingirakamaro mu gusesengura poroteyine no kumenya aho aside amine ziri mu muyoboro wa polypeptide.

Indi mikoreshereze y'ingenzi ya Proteinase K ni ugukuraho ubwandu ku buso. Ikunze gukoreshwa muri laboratwari za molekile mu gukuraho aside nucleic, poroteyine, n'ibindi bihumanya ikirere mu bikoresho bya laboratwari no ku buso bwayo. Proteinase K irahamye kandi ikora neza mu bihe bitandukanye, harimo ubushyuhe bwinshi n'urugero rwa pH rutandukanye.