5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide nuruvange rukoreshwa mubushakashatsi butandukanye bwibinyabuzima, cyane cyane mugushakisha no kubona amashusho yibikorwa bya enzyme.Ni substrate ishobora kuba hydrolyz na enzymes zihariye, bikavamo kurekura ibicuruzwa byamabara cyangwa fluorescent.
Uru ruganda rusanzwe rukoreshwa mubushakashatsi kugirango hamenyekane ahari nibikorwa bya enzymes nka beta-galactosidase na beta-glucuronidase.Iyi misemburo ikuramo amatsinda ya acetyl na glucosaminide kuva muri substrate, biganisha kuri chromofore yubururu cyangwa icyatsi.
Imiterere yihariye ya 5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide itanga uburyo bworoshye bwo kumenya no kugereranya ibikorwa bya enzyme.Gukoresha muburyo butandukanye bwubushakashatsi, harimo histochemie, immunohistochemie, hamwe nubushakashatsi bushingiye ku ngirabuzimafatizo, byagize uruhare mu gusobanukirwa neza imikorere ya enzyme.