5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide CAS: 4264-82-8

5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide ni ikintu gikoreshwa mu bushakashatsi butandukanye bwa biochemical, cyane cyane mu gupima no kwerekana imikorere ya enzyme. Ni substrate ishobora guhindurwamo hydrolyze na enzymes runaka, bigatuma habaho irekurwa ry'umusaruro w'amabara cyangwa fluorescent.

Iyi enzyme ikunze gukoreshwa mu isuzuma kugira ngo hamenyekane niba hari imikorere ya enzyme nka beta-galactosidase na beta-glucuronidase. Izi enzyme zica amatsinda ya acetyl na glucosaminide mu gice cy’umubiri, bigatuma habaho chromophore y’ubururu cyangwa icyatsi.

Imiterere yihariye ya 5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide ituma byoroshye kubona no gupima imikorere ya enzyme. Ikoreshwa ryayo mu buryo butandukanye bwo kugerageza, harimo histochemistry, immunohistochemistry, no gupima imikorere y’uturemangingo, byafashije gusobanukirwa neza imikorere ya enzyme.

Gutumiza cyangwa Gusaba Igiciro

Ibisobanuro birambuye ku gicuruzwa

Ibirango by'ibicuruzwa

Ishyirwa mu bikorwa n'ingaruka

5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide (X-Gluc) ni substrate ya chromogenic ikunze gukoreshwa mu gupima imikorere ya beta-glucuronidase (GUS). GUS ni enzyme iboneka mu binyabuzima bitandukanye, harimo bagiteri, ibimera, n'inyamabere. X-Gluc ikunze gukoreshwa mu isuzuma rya GUS n'ubushakashatsi ku binyabuzima bya molekile.

Ikoreshwa ry'ingenzi rya X-Gluc ni mu buryo bwo gusiga irangi rya histochemical, aho rishobora kwerekana imikorere n'imikorere ya enzyme ya GUS. Iyi substrate iragenda neza mu turemangingo kandi ihinduka hydrolyzed na GUS, bigatuma habaho ubururu butose cyangwa ikintu kidashonga. Iyi rangi ry'ubururu rituma abashakashatsi bashobora kumenya no kumenya imikorere ya GUS mu turemangingo, mu ngingo, no mu binyabuzima byose.

X-Gluc ishobora kandi gukoreshwa mu bipimo by'ingano kugira ngo ipime imikorere ya enzyme ya GUS. Ubukana bw'ibara ry'ubururu cyangwa ingano y'ibicuruzwa byakozwe bishobora kujyana n'urwego rw'imikorere ya GUS cyangwa imikorere yayo ya enzyme.

Byongeye kandi, X-Gluc yakoreshejwe mu bushakashatsi ku bijyanye n’imiterere y’uturemangingo tw’ibimera kugira ngo yige uburyo tugaragara, imikorere y’uturemangingo, n’impinduka z’ibimera. Yanakoreshejwe mu buryo bwa bagiteri mu gukora no gutahura poroteyine za GUS fusion.

Gupakira ibicuruzwa:

Amakuru y'inyongera:

Imiterere	C16H18BrClN2O6
Isuzuma	99%
Isura	Ifu y'umweru
Nimero ya CAS	4264-82-8
Gupakira	Bito kandi byinshi
Igihe cyo kuruhuka	Imyaka 2
Ububiko	Bika ahantu hakonje kandi humutse
Icyemezo	ISO.