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Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside CAS: 24404-53-3
Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside ni uruganda rukunze gukoreshwa mubushakashatsi bwibinyabuzima.Nuburyo bwahinduwe bwisukari ya molekile ya galaktose, kandi ifite porogaramu nyinshi mugusuzuma enzyme, gusesengura imiterere ya gene, sisitemu yo gusuzuma, no kweza poroteyine.Imiterere yacyo irimo amatsinda ya acetyl hamwe nitsinda rya thio, rifasha mukumenya no gukoresha ibikorwa byimikorere idasanzwe.Muri rusange, iyi nteruro ni ingenzi mu kwiga ibikorwa n'imikorere ya enzyme β-galactosidase, ndetse no mubinyabuzima bitandukanye bya molekuline hamwe nubushakashatsi bwibinyabuzima.
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Tris maleate CAS: 72200-76-1
Tris maleate nuruvange rwimiti rukora nka pH buffer noguhindura inganda zitandukanye.Ikoreshwa mukubungabunga urwego pH ruhamye no kurwanya impinduka ziterwa no kongeramo aside cyangwa shingiro.Tris maleate isanzwe ikoreshwa mubushakashatsi bwibinyabuzima, kweza poroteyine, gutunganya inganda, na chimie yisesengura.Ifite akamaro cyane cyane muri buffering kurwego rwo hasi rwa pH kandi irazwi cyane muburyo butandukanye mukubungabunga ibihe byiza bya pH.
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DAOS CAS: 83777-30-4 Igiciro cyabakora
N-Ethyl-N- (2-hydroxy-3-sulfopropyl) -3,5-dimethoxyaniline umunyu wa sodium ni uruganda rwimiti ruri murwego rwa aniline sulfonate.Nuburyo bwumunyu wa sodium, bivuze ko ari muburyo bwa kristaline ikomeye ibora mumazi.Uru ruganda rufite formulaire ya C13H21NO6SNa.
Ifite amatsinda ya alkyl na sulfo, bigatuma agira akamaro mubikorwa bitandukanye.Bikunze gukoreshwa nk'irangi hagati mu gukora amarangi kama, cyane cyane ayakoreshwa mu nganda z’imyenda.Uru ruganda rutanga ibara kandi rutezimbere irangi ryamabara, ryongera imikorere nigihe kirekire.
Byongeye kandi, irashobora kandi gukora nka surfactant bitewe nitsinda ryayo rya hydrophilique sulfonate hamwe na hydrophobique alkyl.Iyi mitungo ituma igabanya ubukana bwamazi hejuru yamazi, ikagira agaciro mumashanyarazi, stabilisateur ya emulsiyo, nibindi bikorwa byinganda zirimo gukwirakwiza ibintu.
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2-hydroxy-4-morpholinepropanesulphonic aside CAS: 68399-77-9
2-hydroxy-4-morpholinepropanesulphonic aside (CAPS) ni agent ya zwitterionic ikoreshwa cyane mubushakashatsi bwibinyabuzima bwa biohimiki na molekile.Nibikorwa byiza bya pH, bikomeza pH ihamye hafi ya 9.2-10.2.CAPS izwi cyane mubikorwa byayo mu kweza poroteyine, gusesengura imisemburo, itangazamakuru ry’umuco, hamwe na electrophorei.Ihuza na enzymes kandi ikoreshwa kenshi mukubungabunga pH nziza kubikorwa bya enzyme mugihe cya laboratoire zitandukanye.CAPS ikoreshwa kandi mubitangazamakuru byumuco w'akagari kugirango habeho ibidukikije byiza byo gukura no kubaho neza.Muri electrophoreis, ifasha kugumya pH ihamye ikenewe mugutandukanya no gusesengura acide nucleic cyangwa proteyine.
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beta-d-glucose pentaacetate CAS: 604-69-3
Beta-D-glucose pentaacetate ni imiti ikomoka kuri glucose, isukari yoroshye.Ikorwa na acetylating glucose hamwe nitsinda rya acetyl eshanu, bikaviramo guhuza ayo matsinda mumatsinda ya hydroxyl (OH) igaragara muri molekile ya glucose.Ihinduka rya glucose ritanga imbaraga zihamye kandi rigashonga mumashanyarazi.
Beta-D-glucose pentaacetate ifite porogaramu zitandukanye mubijyanye na chimie organic, cyane cyane muguhindura no guhindura karubone.Irashobora gukora nkibibanziriza cyangwa hagati mugutegura ibindi bikomoka kuri karubone cyangwa ibinyabuzima bigoye.Byongeye kandi, yakoreshejwe mubikoresho bimwe na bimwe byubuvuzi na farumasi nka sisitemu yo gutanga imiti hamwe nuburyo bwo kurekura.
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3-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE URUBANZA: 3150-25-2
3-Nitrophenyl-beta-D-galactopyranoside (ONPG) ni substrate ikunze gukoreshwa mubushakashatsi bwimisemburo kugirango tumenye kandi dupime ibikorwa bya beta-galactosidase.Iyo beta-galactosidase ihari kandi ikora, itanga hydrolyzes ONPG, ikarekura ibicuruzwa byamabara yumuhondo bita 3-nitropenol.Ubwinshi bwamabara yumuhondo yakozwe burashobora gupimwa spekitifotometometrike, bigatuma umubare wibikorwa bya beta-galactosidase.ONPG ikoreshwa cyane mubinyabuzima bwa molekuline no mubushakashatsi bwa mikorobi, ndetse no mugupima kwa muganga, kugirango bige imvugo ya gene, imikorere ya poroteyine, kumenya bagiteri, hamwe nubuzima bwa selile.
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URUBUGA RWA TOOS: 82692-93-1 Igiciro cyabakora
Sodium 3- (N-ethyl-3-methylanilino) -2-hydroxypropanesulfonate ni imiti ivanze ikunze kwitwa MESNa.Ikoreshwa cyane cyane nkibintu bigabanya ibinyabuzima na biyolojiya.MESNa ifite ubushobozi bwo guca disulfide ihuza poroteyine, ikayihindura mumatsinda ya sulfhydryl.Iyi nzira yo kugabanya ningirakamaro mubikorwa bitandukanye nko gutandukanya poroteyine, kwirinda gukusanya poroteyine, kuranga poroteyine, no kongera poroteyine.MESNa igira uruhare runini mugukoresha poroteyine, gusesengura, no guhindura mubushakashatsi bwa siyansi.
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PIPES umunyu wa monosodium CAS: 10010-67-0
Sodium hydrogen piperazine-1,4-diethanesulphonate, izwi kandi nka HEPES-Na, ni imiti ikoreshwa cyane mu bushakashatsi bw’ibinyabuzima na biohimiki.Ifasha kugumana pH ihamye ya 6.8 kugeza 8.2 mubikorwa bitandukanye, harimo umuco w'akagari, imisemburo ya enzyme, hamwe na tekinoroji ya biologiya.HEPES-Na ihujwe na sisitemu zitandukanye yibinyabuzima kandi irahagaze hejuru yubushyuhe bugari.
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PHENYL-1-THIO-β-D-GALACTOPYRANOSIDE URUBANZA: 16758-34-2
PHENYL-1-THIO-β-D-GALACTOPYRANOSIDE, izwi kandi nka phenyl thio galactopyranoside, ni imiti y’imiti ikomoka mu muryango wa glycoside.Nibikomoka kuri galactose igizwe nigice cyisukari ya galactopyranose ifatanye nitsinda rya fenylthio kuri karubone ya anomeric. Uru ruganda rukunze gukoreshwa mubushakashatsi bwibinyabuzima nka substrate yimisemburo ihuza hydrolyze glycosidic.Ikora nka substrate artificiel yiga ibikorwa byimisemburo ya glycosidase no kumenya umwihariko wabyo, kinetics, no kubuza.PENYL-1-THIO-β-D-GALACTOPYRANOSIDE ikoreshwa kenshi muburyo bwa colimetric na fluorometric kugirango tumenye ahari cyangwa gupima ibihari ibikorwa bya glycosidase zitandukanye mubitegererezo byibinyabuzima.Hydrolysis yuru ruganda rwimisemburo yihariye itanga ibimenyetso byamenyekanye bishobora kugereranywa. Bitewe nitsinda ryayo rihamye rya fenilthio, PHENYL-1-THIO-β-D-GALACTOPYRANOSIDE irashobora gukemurwa byoroshye kandi ikabikwa nta kubora, bigatuma ihitamo neza kuri enzyme isuzuma nubushakashatsi.
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2- (tris (hydroxymethyl) methylamino) Ethane-1-acide sulfonike CAS: 7365-44-8
2--Nka acide sulfonique ikomoka kuri tris (hydroxymethyl) aminomethane (Tris), TES irashobora gukomeza pH ihagaze neza ya 6.8 kugeza 8.2.Ikoreshwa cyane mubitangazamakuru byumuco utugari, reaction enzymatique, protein electrophoreis, nubushakashatsi bwa ADN / RNA.TES irahuze cyane kandi ningirakamaro mugukomeza ibihe byiza mubushakashatsi bwa laboratoire.
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Dipso sodium CAS: 102783-62-0 Igiciro cyabakora
3- [N, N-Bis (hydroxyethyl) amin]Nibikomoka kuri acide sulfonique ikomoka kumunyu wa sodium, bigatuma ibora amazi kandi igahinduka mubisubizo byamazi.
Umunyu wa sodium ya BES ufite molekuline ya C10H22NNaO6S hamwe nuburemere bwa molekile hafi 323.34 g / mol.Bikunze gukoreshwa nkibikoresho byoherejwe bitewe nubushobozi bwayo bwo gukomeza urwego pH ruhamye mubisubizo.
Uru ruganda ruzwiho ubushobozi buhebuje bwo kurwanya impinduka za pH ziterwa no guhindagurika cyangwa kongeramo aside na base.Bikunze gukoreshwa muburyo bwa biologiya na enzymatique, itangazamakuru ryumuco utugari, kweza poroteyine, nibindi bikorwa aho kugenzura neza pH ari ngombwa.
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D - (+) - Cellobiose CAS: 528-50-7
D - (+) - Cellobiose ni disaccharide igizwe nibice bibiri bya glucose bihujwe na beta-1,4-glycosidic.Bikunze kuboneka muri selile, igice cyingenzi cyurukuta rwibimera.Cellobiose nikibara kitagira ibara, kristaline ikomeye ibora mumazi.Ntabwo igogorwa nibinyabuzima byinshi, ariko irashobora guhindurwa na hydrolyz na enzymes zimwe na zimwe nka selobiase, kugirango itange glucose.Cellobiose ni intera ikomeye hagati ya mikorobe yangirika ya selile kandi ikoreshwa mubikorwa bitandukanye bikoresha ikoranabuhanga, harimo no gukora ibicanwa.
