3-NITROPHENYL-BETA-D-GALACTOPIRANOSIDE CAS:3150-25-2

Gutahura imikorere ya beta-galactosidase: ONPG ikunze gukoreshwa mu kumenya niba beta-galactosidase ihari n'imikorere yayo mu bipimo bitandukanye by'ibinyabuzima, nko mu miterere ya bagiteri cyangwa muri lysates z'uturemangingo. Ikorwa rya o-nitrophenol, ifite ibara ry'umuhondo, rishobora gupimwa byoroshye hakoreshejwe spectrophotometer.

Inyigo ku mikorere ya Gene: ONPG ikunze gukoreshwa mu bushakashatsi bwa biology ya molekile kugira ngo yige imikorere ya Gene. Mu guhuza igikoresho gitera inkunga gene ishimishije na gene ikora beta-galactosidase, abashakashatsi bashobora gupima imikorere y'iyi promoter bongeramo ONPG kandi bagapima umusaruro wa o-nitrophenol uvamo. Ubu buryo, buzwi nka beta-galactosidase reporter assay, butanga amakuru ku mikorere ya gene.

Kumenya bagiteri: Bagiteri zimwe na zimwe zikora beta-galactosidase, mu gihe izindi zitabikora. ONPG ishobora gukoreshwa hamwe n'ibindi bipimo bya biochemical kugira ngo hamenyekane ubwoko bwa bagiteri hashingiwe ku bushobozi bwazo bwo gukamura ONPG. Ubu buryo bukunze gukoreshwa mu gusuzuma indwara no muri laboratwari za mikorobe.

Gusuzuma imisemburo ibuza cyangwa ikora ibikorwa: ONPG ishobora gukoreshwa mu gusuzuma imisemburo ihindura imikorere ya beta-galactosidase. Mu gupima imikorere ya enzyme imbere y’imisemburo itandukanye, abashakashatsi bashobora kumenya imisemburo ishobora gutuma ikora ibikorwa bishobora gukorwaho ubushakashatsi ku bushobozi bwayo bwo kuvura.