IMIYOBORO CAS: 5625-37-6 Igiciro cy'uruganda

IMIYOBORO (aside piperazine-1,4-bisethanesulfonic) ni ikintu gikoreshwa mu gufunga zwitterionic gikoreshwa cyane cyane mu bushakashatsi ku binyabuzima no ku binyabuzima. Gifite ibintu byinshi by'ingenzi n'ikoreshwa ryacyo, birimo:

Umuti ugabanya pH: PIPES ni umuti ufasha mu kubungabunga pH ihamye mu bushakashatsi butandukanye bwa biyoloji. Ukoreshwa cyane mu mikorere y'uturemangingo, mu gusuzuma enzymes, no mu mikorere ya molekile.

Ubushobozi bwo gufunga cyane: IMIPAYI ifite ubushobozi bwo gufunga neza mu rugero rwa pH ruri hagati ya 6.1 na 7.5, bigatuma ikoreshwa mu kubungabunga imiterere ya pH ihamye mu buryo butandukanye bw’ibinyabuzima.

Imikoranire mito n'ibinyabutabire: PIPES izwiho kuba idafite aho ihuriye n'imikorere ya biochemical kandi ikaba idahuza na poroteyine na enzymes, bigatuma iba nziza cyane mu kubungabunga ubusugire n'imikorere ya biomolecules.

Ikwiriye ibizamini bishingiye ku bushyuhe: PIPES irashobora kugumana ubushobozi bwayo bwo gufunga mu bushyuhe bwinshi, harimo ubushyuhe bw'umubiri n'ubushyuhe buri hejuru. Ibi bituma ikwiriye igerageza risaba gutuza no gukora neza mu bihe bitandukanye by'ubushyuhe.

Uburyo bwo gukoresha Electrophoresis: IMIYOBORO ikunze gukoreshwa nk'uburyo bwo gukingira amashanyarazi mu buryo bwa gel electrophoresis, nka RNA cyangwa DNA agarose gel electrophoresis, bitewe nuko ifite ubushobozi buke bwo kwinjiza UV n'ubushobozi bwo gutwara amashanyarazi bwinshi.

Imiti ikoreshwa mu gukora imiti: PIPES ikoreshwa kandi mu gukora imiti nk'umuti urinda indwara, igatanga ubusugire no kubungabunga pH nziza kugira ngo imiti ikore neza.