Fenilgalactoside CAS: 2818-58-8

Ingaruka kubikorwa bya enzyme: Phenylgalactoside ikoreshwa mugupima ibikorwa bya enzyme β-galactosidase.Iyo fenylgalactoside ihinduwe na hyd-galactosidase, irekura p-nitropenol.Ikusanyirizo rya p-nitropenol irashobora gupimwa mubwinshi, itanga ubushishozi mubikorwa bya β-galactosidase.Ingaruka ikoreshwa mubisabwa nka enzyme yerekana na sisitemu yo gusuzuma.

Isesengura ry'imiterere y'ibisekuru: Phenylgalactoside ikoreshwa nka substrate mubushakashatsi bwibinyabuzima bwa molekuline kugirango bige imvugo ya gene.Gene ya lacZ, ikubiyemo β-galactosidase, ikunze guhuzwa nuburyo bukurikirana bwizindi genes zinyungu.Imvugo ya gene ya lacZ na hydrolysis ya fenylgalactoside na β-galactosidase irashobora kwerekana imiterere nurwego rwa gen igamije kwigwa.

Sisitemu yo gusuzuma: Phenylgalactoside nikintu cyingenzi cya sisitemu yo gusuzuma ikoresha β-galactosidase.Urugero rumwe ruzwi cyane nuburyo bwo gusuzuma ubururu-bwera, bukoreshwa mukumenya ingirabuzimafatizo cyangwa zahinduwe mubushakashatsi bwibinyabuzima bwa molekile.Ubukoloni bwafashe neza ADN ya recombinant cyangwa bwakorewe genetique bizagaragaza β-galactosidase, biganisha kuri hydrolysis ya fenylgalactoside no gukora ibara ry'ubururu.

Isuku rya poroteyine: Rimwe na rimwe, fenylgalactoside irashobora gukoreshwa nka ligand ya chromatografi ya affinity kugirango isukure poroteyine zihuza cyane cyangwa zikorwa na β-galactosidase.Poroteyine yinyungu irashobora kugira ikirangantego cyangwa ikirango cyo guhuza kirimo β-galactosidase-ihuza indangarubuga.Iyo unyuze kuri poroteyine ivanze unyuze mu nkingi hamwe na fenylgalactoside itabangamiwe, poroteyine yifuzwa irashobora kugumana guhitamo hanyuma igatorwa nyuma.