NSP-SA-NHS CAS:199293-83-9 Igiciro cy'umukozi

NSP-SA-NHS, izwi kandi nka N-succinimidyl S-acetylthioacetate N-hydroxysuccinimide ester, ni ikintu gikunze gukoreshwa nk'ikintu kivangavanga thiol mu buryo bw'imikorere ya bioconjugation. Ingaruka zayo nyamukuru ni ukurema imigozi ihamye ya thioester hagati y'amatsinda ya thiol ari kuri biomolecules, nka poroteyine cyangwa peptides.

Gukoresha NSP-SA-NHS ahanini ni mu rwego rwo guhindura poroteyine no kuyihagarika. Zimwe mu nshingano zayo z'ingenzi zirimo:

Gushyira ibirango bya poroteyine ku bipimo: NSP-SA-NHS ikoreshwa mu guhuza ibirango, nka amarangi ya fluorescent cyangwa biotin, kuri poroteyine cyangwa peptides. Ibi bituma hamenyekana, hakamenyekana, kandi hagakurikiranwa biomolecules zanditseho ibipimo mu bipimo bitandukanye by’ibinyabuzima.

Uburyo poroteyine ikorana: NSP-SA-NHS ishobora gukoreshwa mu guhuza poroteyine zikorana kugira ngo yige uburyo poroteyine ikorana. Ibi bikunze gukoreshwa mu buryo nko guhuza immunoprecipitation cyangwa ibizamini byo gukuraho kugira ngo hamenyekane abafatanyabikorwa cyangwa imiterere ya poroteyine ikoreshwa mu kwiga.

Kubuza poroteyine kugenda: NSP-SA-NHS yemerera poroteyine cyangwa peptides gufatana ku buso bukomeye, harimo agarose beads, magnetic beads, cyangwa microplates. Ibi ni ingirakamaro mu bikorwa nko gusukura affinity, gusuzuma imiti, cyangwa guteza imbere biosensors.

Guhindura ubuso: NSP-SA-NHS ishobora gukoreshwa mu guhindura ubuso, nk'udupira tw'ikirahure cyangwa uduce duto, hamwe na poroteyine cyangwa peptides, bigatuma ubuso bukoreshwa mu gukora ibintu bitandukanye nko gusuzuma, uburyo bwo gutanga imiti, cyangwa uburyo bwo kumenya ibintu.