N,N'-Methylenebisacrylamide CAS:110-26-9

N,N'-Methylenebisacrylamide (MBAA) ni ikinyabutabire gikunze gukoreshwa mu bushakashatsi bwa molekile na biochimie. Ni ikintu gihuza ibyuma gishyirwa muri acrylamide gels kugira ngo kibe polimeri kandi gikore matrix ihamye yo gukoresha electrophoresis.

Guhuza ibyuma bivuga ishyirwaho ry'imigozi ya shimi hagati y'imigozi ya polymer, kandi MBAA igira uruhare runini muri iki gikorwa. Iyo yongewe kuri acrylamide monomers, MBAA irazihuza kugira ngo zikore urusobe rw'ibice bitatu bya polymer. Iyi miterere ihuza ibyuma yongera ubusugire n'imbaraga bya gel, bigatuma biomolecules zitandukanywa hashingiwe ku bunini bwazo, imbaraga, cyangwa indi miterere yazo.

MBAA ikoreshwa cyane cyane mu buryo nka polyacrylamide gel electrophoresis (PAGE) na sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), ikoreshwa cyane mu gusesengura poroteyine na aside nucleic. Ifasha mu gukora matrix ya gel imwe aho biomolecules zishobora kwimuka mu gihe cya electrophoresis.

Uretse ikoreshwa ryayo muri electrophoresis ya gel, MBAA inasangamo n'izindi nzego nko gushyira mu bikorwa molekile, chromatography, na sisitemu yo gutanga imiti. Izwiho ubushobozi bwo gukora imiyoboro ya polymer ihamye kandi ikomeye mu buryo bwa mekanike, bigatuma iba igikoresho cy'agaciro mu bushakashatsi no muri laboratwari zisesengura.

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Ishyirwa mu bikorwa n'ingaruka

Ingaruka zo guhuza: MBAA ishobora guhuza acrylamide monomers kugira ngo ikore imiyoboro ya polymer ihuza. Iyi ngaruka yo guhuza yongera ubushobozi n'imbaraga bya gels cyangwa polymer matrices.

Gel Electrophoresis: Imwe mu mikoreshereze ikunze gukoreshwa ya MBAA ni muri tekiniki za gel electrophoresis nka polyacrylamide gel electrophoresis (PAGE) na sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). MBAA yongerwa kuri acrylamide monomers kugira ngo izihuze kandi zikore matrix ya gel ihamye, bigatuma biomolecules zitandukana bitewe n'ingano yazo n'umuvuduko wazo mu gihe cya electrophoresis.

Isesengura rya Poroteyine: MBAA ni ingenzi mu bushakashatsi no gusesengura poroteyine. Yemerera gutandukanya poroteyine ukurikije ingano yayo kandi igatuma Western blotting, protein cleansing, n'ubundi buryo bwo kugena poroteyine.

Isesengura rya aside ya nucleique: MBAA ikoreshwa kandi mu buryo bwo gusesengura aside ya nucleique nko gusesengura ADN, gutandukanya ibice bya ADN, no gupima ADN. Itanga gel matrix ihamye yoroshya gutandukanya ibice bya ADN hashingiwe ku bunini bwabyo.

Gucapa Molecular: MBAA ikoreshwa mu buryo bwo gucapa molecular, aho molecular template ishyirwa muri polymer matrix. Nyuma yo gukuraho template, polymer matrix igumana kwibuka imiterere ya molecular, bigatuma ifatana neza kandi ikamenyekana.

Sisitemu zo Gutanga Imiti: MBAA ishobora gukoreshwa mu gukora imiyoboro ya polymer ihuza hamwe ifite ubushobozi bwo kurekura imiti cyangwa izindi molekile mu buryo bugenzurwa. Ifite akamaro mu gukora imiti ikomeza kurekura imiti, kongera ubushobozi bwo kugumana imiti, no kunoza imikorere myiza yo gutanga imiti.

Gupakira ibicuruzwa:

Amakuru y'inyongera:

Imiterere	C7H10N2O2
Isuzuma	99%
Isura	Ifu y'umweru
Nimero ya CAS	110-26-9
Gupakira	Bito kandi byinshi
Igihe cyo kuruhuka	Imyaka 2
Ububiko	Bika ahantu hakonje kandi humutse
Icyemezo	ISO.