Nitrotetrazolium Ubururu bwa kloride CAS: 298-83-9

Mu bushakashatsi ku binyabuzima no ku binyabuzima, Nitrotetrazolium Blue chloride ikora nk'igikoresho cy'ingirakamaro mu gupima no gupima superoxide radicals, igira uruhare runini mu mikorere itandukanye y'umubiri n'indwara. Iyo superoxide ikoreshejwe, NBT ikora umusaruro w'ubururu udashonga wa formazan, bigatuma abashakashatsi bashobora gusuzuma neza niba superoxide iriho kandi ikwirakwira mu turemangingo tw'uturemangingo, mu ngingo, cyangwa mu binyabuzima byose. Ubu bushobozi butuma ubushakashatsi ku ruhare rwa superoxide radicals mu bintu bitandukanye by'ibinyabuzima, harimo gutanga ibimenyetso ku turemangingo, uburyo bwo kwirinda indwara, no kwangirika kwa oxidative. Uburyo NBT ikoresha superoxide radicals butanga ubumenyi ku ruhare rw'ubwoko bwa ogisijeni mu buzima no mu ndwara, butanga uburyo bwo gusuzuma indwara ziterwa n'ubwonko nk'indwara z'umutima, indwara z'ubwonko, n'ingaruka zo kubyimba. Byongeye kandi, akamaro ka NBT kagera no ku isuzuma ry'ikorwa rya superoxide mu buryo bw'igerageza bw'indwara, bufasha mu gusobanukirwa uburyo indwara ziterwa n'ubwonko no guteza imbere uburyo bushobora gukoreshwa mu kuvura bugamije inzira zo kubyimba. Byongeye kandi, ikoreshwa rya NBT ntirigarukira ku bushakashatsi bw'ibanze gusa, kuko rifite ingaruka mu gusuzuma indwara, cyane cyane mu isuzuma ry'imikorere y'ubudahangarwa bw'umubiri n'imiterere y'ububyimba. Mu kwiyumvisha ikorwa rya superoxide hakoreshejwe NBT, abashakashatsi n'abaganga bashobora kubona amakuru y'ingenzi ku bikorwa by'uturemangingo tw'umubiri n'imikorere idahwitse ya oxidative ijyanye n'indwara zitandukanye, bityo bigafasha gusuzuma no gupima. Muri make, Nitrotetrazolium Blue chloride igira uruhare runini mu bushakashatsi bw'ibinyabuzima n'ibijyanye na biochemical, ikaba igikoresho gifite akamaro kanini mu gushakisha no kwerekana superoxide radicals. Ubushobozi bwayo bwo gukora formazan y'ubururu yihariye nyuma yo gukora hamwe na superoxide bufasha iperereza ku muvuduko wa oxidative, ibisubizo by'ubudahangarwa bw'umubiri, n'imiterere y'indwara. Gukoresha NBT bifasha mu gusobanukirwa byimbitse uruhare rw'ubwoko bwa ogisijeni ikora mu buzima n'indwara, hamwe n'ingaruka zishobora guterwa no guteza imbere ibikoresho byo gusuzuma no kuvura hibandwa ku ndwara ziterwa na oxidative stress. Ubushakashatsi bukomeje no gukoresha NBT bishobora gutanga ubumenyi bwinshi ku kamaro kayo kandi bigafasha mu iterambere mu gusobanukirwa ibinyabuzima bya redox n'izindi nzira zijyanye nabyo.