Hari intwari nyinshi zitavugwa zituzengurutse, zisa n'izisanzwe, ariko mu by'ukuri zidukorera byinshi bucece. Proteyine K ni "intwari itavugwa" mu nganda zisuzuma ingirabuzimafatizo, nubwo ugereranije n' "inini kandi ikomeye" mu nganda, poroteyine K ni nto cyane ku buryo tumaze igihe twirengagije akamaro kayo. Nyuma y'icyorezo gishya cy'ikamba, ubwinshi bwa poroteyine K bwariyongereye, kandi ikoreshwa mu gihugu no mu mahanga riri inyuma cyane y'ikoreshwa, kandi buri wese yahise abona ko poroteyine K ari ingenzi cyane.
Ni iki gikoreshwa muri poroteyine K?
Proteyine K ni serine protease ifite imikorere ya enzyme ya proteolytic kandi ishobora gukomeza gukora ahantu hatandukanye (pH (4-12.5), ububiko bw'umunyu mwinshi, ubushyuhe bwinshi bwa 70°C, nibindi). Byongeye kandi, imikorere ya proteinase K ntibuzwa na SDS, urea, EDTA, guanidine hydrochloride, guanidine isothiocyanate, nibindi, kandi ingano runaka y'isabune ishobora no kongera imikorere ya proteinase K. Mu buvuzi (virusi n'imiti yica udukoko), ibiryo (gukora inyama neza), uruhu (kworoshya umusatsi), gukora divayi (kugabanya inzoga), gutegura aside amine (ubwoya bwangiritse), gukuramo aside nucleic, guhuza imiterere y'umubiri, nibindi, proteinase K Hari uburyo bwo kuyikoresha. Uburyo bukunze gukoreshwa ni ugukuramo aside nucleic.
Proteyine K ishobora gukora enzyme ya poroteyine zose ziri mu gipimo, harimo na za histones zifatanye neza na aside nucleic, kugira ngo aside nucleic zisohoke mu gipimo hanyuma zisohoke mu gipimo, byorohereze intambwe ikurikira yo gukuramo no gutunganya. Mu gutahura aside nucleic ya virusi, poroteyine K ni kimwe mu bintu by'ingenzi mu gisubizo cyo gufata ingero za virusi. Proteyine K ishobora guca no guhagarika poroteyine ya virusi, ikaba yizewe mu gihe cyo kuyitwara no kuyimenya; byongeye kandi, poroteyine K ishobora no kwangiza RNase irinda kwangirika kwa RNA ya virusi kandi ikoroshya kuvumbura aside nucleic.
Imenyekana rya poroteyine K mu ijoro rimwe
Haba mu rwego rw'ubushakashatsi bwa siyansi cyangwa mu rwego rwa IVD, gukuramo aside nucleic ni cyo gikorwa cy'ibanze, bityo poroteyine K yahoraga ari ikintu cy'ingenzi cyane. Ariko, mu bihe byashize, poroteyine K ntiyari izwi cyane nk'uruhare rwayo. Igice kinini cy'ibi cyatewe nuko isano iri hagati ya poroteyine K n'ibikenewe yari ihamye cyane. Abantu bake ni bo batekereza ko poroteyine K yaba ikibazo.
Kubera icyorezo gishya cya korona cyatangiye, icyifuzo cyo gupima aside nucleic cyariyongereye cyane. Mu mpera za Kamena 2020, Ubushinwa bwarangije ibizamini bishya bya korona bigera kuri miliyoni 90, kandi uyu mubare urushaho gutera ubwoba ku rwego rw'isi. Mu igerageza ryo gukuramo aside nucleic, ingano ya poroteyine K ikora ni hagati ya 50-200 μg / mL. Muri rusange, bisaba μg 100 za poroteyine K kugira ngo hakorwe icyitegererezo cya aside nucleic. Mu ikoreshwa nyaryo, kugira ngo hongerwe imikorere yo gukuramo aside nucleic, akenshi Proteyine K izakoreshwa mu bwinshi. Gutahura aside nucleic ya coronavirus nshya byatumye habaho ingano nini ya poroteyine K. Ingano y'ibanze y'ibikenewe bya poroteyine K yahungabanye vuba, maze poroteyine K iba igikoresho cy'ingenzi cyo gukumira icyorezo mu ijoro rimwe.
Ingorane mu ikorwa rya poroteyine K
Nubwo icyorezo cyateye imbere, agaciro ka poroteyine K kagiye gahabwa agaciro n'abantu, biteye isoni kubona kubera ko poroteyine K nke cyane, ibigo bike byo mu gihugu byagize uruhare mu ikorwa rya poroteyine K. Iyo abantu bashaka gushyiraho poroteyine K. Mu gihe cyo gukora, byagaragaye ko poroteyine K ari poroteyine idasanzwe cyane. Biragoye cyane kwagura ubushobozi bwo gukora poroteyine K mu gihe gito.
Ikorwa rya poroteyine K ku rwego runini rihura n'ingorane zikurikira
1. Kugaragara nabi kw'isura
Proteyine K ishobora kwangiza poroteyine nyinshi mu buryo butari bwihariye kandi igatera uburozi bukomeye ku turemangingo tw’umubiri. Kubwibyo, urwego rw’imikorere ya poroteyine K muri rusange ruba ruri hasi cyane. Gusuzuma sisitemu zo kugaragaza imiterere n’ubwoko bwa poroteyine K muri rusange bisaba igihe kirekire.
2. Ibisigazwa by'amabara na aside nucleike
Gutunganya cyane bitanga ibara ryinshi ry’ibisigazwa bya aside nucleique. Biragoye gukuraho iyo myanda hakoreshejwe uburyo bworoshye bwo kuyisukura, kandi kuyisukura mu buryo bugoye byongera ikiguzi kandi bikagabanya igipimo cyo kuyigarura.
3. Kudahinduka
Proteyine K ntabwo ihamye bihagije, ishobora kwiyinjizamo enzyme, kandi biragoye kuyibika neza kuri dogere selisiyusi 37 igihe kirekire nta kintu kiyirinda.
4. Byoroshye kugusha neza
Mu gutegura ifu yumye ya poroteyine K ifu ifumye, kugira ngo ingano ikomeye ya poroteyine K mu ifu yumye ifumye ari nini, ni ngombwa kongeramo ikintu kiyirinda yumye ifumye ku rugero rwo hejuru, ariko iyo ingano ya poroteyine K igeze kuri 20mg/mL no hejuru yayo, biroroshye. Guteranya ibintu bitera impanuka, bigatera ingorane zikomeye mu kumisha poroteyine K ifumye ifite ingano ikomeye.
5. Ishoramari rinini
Proteyine K ifite imikorere ikomeye ya poroteyine kandi ishobora gukurura izindi poroteyine muri laboratwari. Kubwibyo, poroteyine K ikenera ahantu hihariye ho gukora, ibikoresho, n'abakozi bo gukora ubushakashatsi, iterambere n'umusaruro.
Umuti wa XD BIOCHEM wa poroteyine K
XD BIOCHEM ifite urubuga rwo gukora no gutunganya poroteyine, kandi ifite ubunararibonye bwinshi mu gukora no gutunganya poroteyine zihuza ibidukikije no kunoza uburyo bwo gukora. Binyuze mu ishyirwaho ryihuse ry'itsinda ry'ubushakashatsi n'iterambere, inzira nini yo gukora poroteyine K yaratsinzwe. Umusaruro wa buri kwezi w'ifu yumye ifu ifunze urenga 30 KG. Iki gicuruzwa gifite imikorere ihamye, imikorere ya enzyme yihariye, kandi nta bisigazwa bya cytochrome na aside nucleic. Murakaza neza guhamagara XD BIOCHEM Shaka ipaki y'igerageza (Imeri:sales@xdbiochem.comTerefone: +86 513 81163739).
Ibisubizo bya tekiniki bya XD BIOCHEM birimo
Hakoreshejwe uburyo bwo guhuza plasmide bufite kopi nyinshi, hatoranywa ubwoko bw’ibintu bifite urwego rwo kugaragaza ingano ya 8g/L, ibyo bikaba bikemura ikibazo cy’urugero rwo hasi rwa poroteyine K.
Binyuze mu gushyiraho uburyo bwo gusukura mu ntambwe nyinshi, ibisigazwa bya cytochrome na aside nucleic bya proteinase K byakuweho neza munsi y'agaciro gasanzwe.
Binyuze mu gusuzuma uburyo bworoshye bw’imiti irinda indwara, hatoranijwe uburyo bwo kubika poroteyine K mu buryo buhamye ku bushyuhe bwa 37°C.
Uburyo bwo gusuzuma bukemura ibibazo by’uko poroteyine K yoroshye kuyiteranya no kuyiteranya ku bwinshi, kandi bushyiraho urufatiro rwo kumisha poroteyine K mu buryo bukabije.
Icyitegererezo cya XD BIOCHEM poroteyine K
Ikizamini cya XD BIOCHEM proteinase K: nta mpinduka nini izabaho mu mikorere nyuma ya dk 80 ku bushyuhe bw'icyumba
Ikizamini cya XD BIOCHEM proteinase K: nta mpinduka nini izabaho mu mikorere nyuma ya dk 80 ku bushyuhe bw'icyumba.
Kugereranya ingaruka zo gukuramo aside nucleic ya XD BIOCHEM proteinase K n'ibindi bicuruzwa bihanganye. Mu gikorwa cyo gukuramo aside nucleic, XD BIOCHEM na proteinase K bikoreshwa uko bikurikirana. Ubushobozi bwo gukuramo proteinase K ya XD BIOCHEM buri hejuru kandi agaciro ka Ct k'ingirabuzimafatizo kari hasi.
Igihe cyo kohereza: Ukuboza 31-2021