Hano hari intwari nyinshi zitaririmbwe, zisa nkizisanzwe, ariko mubyukuri zidufasha bucece.Proteinase K ni "intwari itavuzwe" mu nganda zipima molekuline, nubwo ugereranije n "" nini kandi ikomeye "mu nganda, proteinase K ni urufunguzo ruto cyane ku buryo twirengagije akamaro kayo.Hamwe n'icyorezo gishya cy’ikamba, icyifuzo cya proteinase K cyiyongereye, kandi itangwa mu gihugu ndetse no hanze yarinyuma cyane yo kurya, abantu bose bahita bamenya ko proteinase K ari ngombwa.
Gukoresha proteinase K ni ubuhe?
Proteinase K ni protease ya serine ifite ibikorwa bya enzyme ya proteolyique kandi irashobora gukomeza ibikorwa mubice bitandukanye (pH (4-12.5), buffer yumunyu mwinshi, ubushyuhe bwinshi bwa 70 ° C, nibindi).Byongeye kandi, ibikorwa bya proteinase K ntibibujijwe na SDS, urea, EDTA, hydrochloride ya guanidine, guanidine isothiocyanate, nibindi, kandi umubare munini wimyenda ishobora kandi kongera ibikorwa bya proteinase K. Mu buvuzi (virusi na mikorobe yanduza mikorobe) ).Porogaramu ikoreshwa cyane ni gukuramo aside nucleic.
Proteinase K irashobora gukora enzymolyze yubwoko bwose bwa poroteyine murugero, harimo naya mateka afitanye isano cyane na acide nucleic, kugirango acide nucleic acide irekurwe hanyuma ikarekurwa mubikuramo, byorohereza intambwe ikurikira yo gukuramo no kwezwa.Mu gutahura aside nucleic aside, proteinase K nikimwe mubice byingenzi mugukemura virusi.Proteinase K irashobora kumenagura no kudakora proteine ya kote ya virusi, ikaba ifite umutekano mugihe cyo gutwara no gutahura;hiyongereyeho, proteinase K irashobora kandi gutesha agaciro RNase irinda kwangirika kwa virusi ya RNA kandi ikorohereza kumenya aside nucleic.
Icyamamare mwijoro rya proteinase K.
Haba mubushakashatsi bwubumenyi cyangwa mubijyanye na IVD, gukuramo aside nucleic nubushakashatsi bwibanze, bityo proteinase K yamye ibaho cyane.Ariko, mubihe byashize, proteinase K ntiyari izwi cyane kurenza uruhare rwayo.Igice kinini cyibi byatewe nuko umubano wo gutanga no gusaba proteinase K wari uhagaze neza.Abantu bake ni bo batekereza ko itangwa rya proteinase K ryaba ikibazo.
Hamwe n'icyorezo gishya cy'ikamba, icyifuzo cyo gupima aside nucleic cyiyongereye.Kugeza mu mpera za Kamena 2020, Ubushinwa bwarangije ibizamini bishya bigera kuri miliyoni 90, kandi iyi mibare irateye ubwoba ku isi yose.Mu bushakashatsi bwo gukuramo aside nucleic, ubushakashatsi bwa proteinase K bugera kuri 50-200 μg / mL.Mubisanzwe, bisaba hafi 100 μg za proteinase K kugirango ukuremo urugero rwa acide nucleic.Mu mikoreshereze nyayo, kugirango twongere imikorere yo gukuramo aside nucleic, akenshi Proteinase K izakoreshwa mubwinshi.Nucleic acide ya coronavirus nshya yazanye proteinase K nyinshi.Isoko ryambere hamwe nibisabwa bya proteinase K byacitse vuba, kandi proteinase K yabaye ibikoresho byingenzi byo gukumira icyorezo ijoro ryose.
Ingorane zo gukora proteinase K.
Nubwo hamwe n’iterambere ry’icyorezo, agaciro kingenzi ka proteinase K kahawe agaciro n’abantu, biteye isoni kubona kubera urufunguzo ruke cyane rwa proteinase K, amasosiyete make yo mu gihugu yagize uruhare mu gukora proteinase K. Iyo abantu ushaka gushyiraho proteinase K Mugihe cyibikorwa, byavumbuwe ko proteinase K ari proteine idasanzwe.Biragoye cyane kwagura ubushobozi bwa proteinase K mugihe gito.
Umusaruro munini wa proteinase K uhura ningorane zikurikira
1. Imvugo mike
Proteinase K irashobora gutesha agaciro poroteyine nyinshi kandi igatera uburozi bukomeye kumagambo yakira selile.Kubwibyo, imvugo urwego rwa proteinase K muri rusange ni hasi cyane.Kugaragaza imiterere ya sisitemu n'imirongo igaragaza proteinase K muri rusange bisaba uruziga rurerure.
2. Ibisigisigi bya pigment na acide nucleic
Ubunini bunini bwa fermentation butangiza umubare munini wa pigment hamwe nibisigisigi bya acide nucleic.Biragoye gukuraho ibyo byanduye hamwe nuburyo bworoshye bwo kweza, kandi kweza bigoye byongera ikiguzi kandi bigabanya igipimo cyo gukira.
3. Guhungabana
Proteinase K ntabwo ihagaze neza bihagije, irashobora enzymolyze ubwayo, kandi biragoye kuyibika neza kuri 37 ° C mugihe kirekire nta muti urinda.
4. Biroroshye kugwa
Mugihe utegura ifu yumye ya proteinase K, kugirango harebwe niba ibintu bikomeye bya proteinase K mu ifu yumye ya feri ari nini, birakenewe ko wongeramo imiti ikingira ikonje cyane, ariko mugihe kwibumbira hamwe kwa proteinase K bigera kuri 20mg / mL no hejuru, biroroshye Igiteranyo gikora imvura, izana ingorane zikomeye mugukonjesha-gukama proteinase K hamwe nibintu bikomeye.
5. Ishoramari rinini
Proteinase K ifite ibikorwa bya protease bikomeye kandi irashobora hydrolyze izindi protease muri laboratoire.Kubwibyo, proteinase K isaba ahantu hihariye ho kubyaza umusaruro, ibikoresho, nabakozi mubushakashatsi niterambere no kubyaza umusaruro.
XD BIOCHEM ya proteinase K igisubizo
XD BIOCHEM ifite poroteyine ikuze kandi yerekana uburyo bwo kweza, kandi ifite uburambe bukomeye mu mvugo no kweza poroteyine za recombinant no kunoza imikorere.Binyuze mu gushiraho byihuse itsinda ryubushakashatsi niterambere, inzira nini yo gukora proteinase K yaratsinzwe.Ibisohoka buri kwezi byifu yumye irenze 30 KG.Igicuruzwa gifite imikorere ihamye, enzyme yo hejuru yibikorwa byihariye, kandi nta cytochrome yakira hamwe na acide nucleic.Murakaza neza kuvugana na XD BIOCHEM Kubona igeragezwa (E-imeri:sales@xdbiochem.comTel: +86 513 81163739).
XD BIOCHEM ibisubizo bya tekiniki birimo
Ukoresheje kopi-kopi ya plasmid ihuza, imvugo-yohejuru cyane hamwe nurwego rwa 8g / L rwatoranijwe, rutsinda ikibazo cyimvugo yo hasi ya proteinase K.
Binyuze mu gushiraho intambwe nyinshi zo kweza, host cytochrome hamwe na aside nucleic ibisigisigi bya proteinase K byavanyweho neza munsi yagaciro gasanzwe.
Binyuze mu bipimo byinshi byerekanwa byerekana uburyo bwo kurinda, buffer ishobora kubika proteinase K kuri 37 ° C yatoranijwe.
Kugenzura buffers byatsinze ikibazo proteinase K yoroshye kwegeranya no kugwa kumurengera mwinshi, kandi igashyiraho urufatiro rwa proteinase K yibintu byinshi bikomeye bikonje-byumye.
XD BIOCHEM proteinase K icyitegererezo
XD BIOCHEM proteinase K ikizamini gihamye: ntihazabaho impinduka zikomeye mubikorwa nyuma ya 80 d mubushyuhe bwicyumba
XD BIOCHEM proteinase K ikizamini gihamye: ntihazabaho impinduka zikomeye mubikorwa nyuma ya 80 d mubushyuhe bwicyumba.
Kugereranya ingaruka zo gukuramo aside nucleic ya XD BIOCHEM proteinase K nibicuruzwa birushanwe.Muburyo bwo gukuramo aside nucleic, XD BIOCHEM hamwe na proteinase K irushanwa ikoreshwa.Gukuramo neza kwa XD BIOCHEM proteinase K iri hejuru kandi Ct agaciro ka gen igenewe ni munsi.
Igihe cyo kohereza: Ukuboza-31-2021