NBD chloride CAS: 10199-89-0

Chloride ya NBD, izwi kandi nka 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole, ni irangi rikoresha fluorescent rikunze gukoreshwa mu bintu bya shimi na biyoloji. Iri mu bwoko bwa nitrobenzoxadiazole (NBD), ifite ubushobozi bukomeye bwo kugaragaza fluorescence.

Chloride ya NBD irangwa n'ubushobozi bwayo bwo gukora imiyoboro ya covalent hamwe na nucleophiles, nka amines z'ibanze, thiols, na hydroxyl. Iyi miterere yemerera gukoreshwa nk'ikintu gitanga ibimenyetso kuri biomolecules, harimo poroteyine, peptides, aside nucleic, n'izindi molekile nto.

Iyo NBD chloride ihuye na nucleophile, ihinduka ikintu gisimbura ibindi, bigatuma habaho ikintu gihamye gifite ikirango cya NBD. Iki gicuruzwa gifite ikirango kigaragaza fluorescence ikomeye, bigatuma kiba ingirakamaro mu buryo butandukanye bwo gushakisha no gufata amashusho.

Isohoka ry'urumuri rw'ibinyabutabire bya NBD rishobora gukururwa n'urumuri rwa ultraviolet (UV) ruri hafi ya 470 nm, kandi urumuri rusohoka rukunze kugaragara hafi ya 530 nm. Ubu bunini bw'urumuri bugaragara mu gace k'icyatsi kibisi, aho kubangamira imiterere y'ibinyabutabire mu buryo bw'ibinyabuzima biri hasi cyane.

Gutumiza cyangwa Gusaba Igiciro

Ibisobanuro birambuye ku gicuruzwa

Ibirango by'ibicuruzwa

Ishyirwa mu bikorwa n'ingaruka

Ibirango bya Covalent: NBD chloride ishobora gukora imigozi ya covalent hamwe n'amatsinda ya nucleophilic mu binyabuzima, nka amines z'ibanze, thiols, na hydroxyl. Ibi bituma hashyirwaho ibirango byihariye by'ibi bintu bya biomolecules kugira ngo bivumburwe kandi bifashishwe mu gushushanya.

Kongera urumuri: Iyo NBD chloride ihuye na nucleophiles, ikora ikintu gifite ikirango cya NBD gikozwe muri fluorescent. Iki gicuruzwa gifite ikirango kigaragaza urumuri rukomeye, bigatuma kiba ingirakamaro mu kureba no gukurikirana molekile zifite ikirango.

Ubushobozi bwo kumva no gutandukanya ibintu: Ingufu zituruka ku bicuruzwa bya NBD zigaragaza imiterere yabyo cyane, bigatuma habaho ingano nto y’ibinyabutabire byanditseho. Byongeye kandi, imiterere y’umugozi hagati ya NBD chloride na nucleophiles itanga imiterere yihariye, kuko ituma irangi rifatana neza kandi mu buryo bwihariye kuri biomolecule.

Ibirango bya biomolecule: NBD chloride ishobora gukoreshwa mu gushyira ibirango bya biomolecule zitandukanye, harimo poroteyine, peptides, aside nucleic, n'utundi tunyangingo duto. Ibi bituma abashakashatsi bashobora gukora ubushakashatsi ku gace, imiterere, n'imikoranire y'izi biomolecule mu buryo bw'ibinyabuzima.

Mikroskopi y'urumuri: Biomolecules zifite ikirango cya NBD zishobora kugaragazwa hakoreshejwe mikroskopi y'urumuri. Ubu buryo butuma umuntu abona molekile zifite ikirango ku rwego rw'uturemangingo cyangwa uturemangingo, bigatanga amakuru y'ingirakamaro ku bijyanye n'uburyo zikwirakwira n'imyitwarire yazo.

Isesengura ry’imiterere y’uturemangingo: Isesengura ry’imiterere y’uturemangingo (biomolecules) rifite ikirango cya NBD rishobora gupimwa no gupimwa hakoreshejwe isesengura ry’imiterere y’uturemangingo. Ubu buryo butuma habaho isesengura ry’uturemangingo cyangwa uduce duto tw’uturemangingo, bigatanga amakuru ajyanye n’imiterere y’uturemangingo cyangwa imiterere y’ubuso bw’uturemangingo.

Gushyira no gukurikirana poroteyine: NBD chloride ishobora gukoreshwa mu gushyira ibisigazwa bya aside amine cyangwa amatsinda imikorere muri poroteyine. Ibi bituma abashakashatsi biga imiterere ya poroteyine, uburyo igenda, imikorere, n'imikoranire mu buryo bufatika.

Gupakira ibicuruzwa:

Amakuru y'inyongera:

Imiterere	C6H2ClN3O3
Isuzuma	99%
Isura	Ifu y'umuhondo
Nimero ya CAS	10199-89-0
Gupakira	Bito kandi byinshi
Igihe cyo kuruhuka	Imyaka 2
Ububiko	Bika ahantu hakonje kandi humutse
Icyemezo	ISO.