Iodoacetamide CAS:144-48-9 Igiciro cy'umukozi

Guhindura Cysteine: Iodoacetamide igira ingaruka ku matsinda ya thiol yigenga mu bisigazwa bya cysteine, igakora isano ihuza na cysteine ​​sulfur. Iyi mpinduka ifasha mu isesengura rya poroteyine ibuza ibisigazwa bya cysteine ​​no gukumira ingaruka mbi cyangwa oxidation.

Igogora rya poroteyine: Iodoacetamide ikunze gukoreshwa mu igogora rya poroteyine mu gusesengura spectrometry y'umubare munini. Ikorana n'ibisigazwa bya cysteine ​​mbere yo gugogora enzyme, ikarinda ishingwa ry'imibanire idakenewe ya disulfide no kugumana poroteyine mu gihe cyayo cyagabanutse.

Ubushakashatsi ku miterere ya Proteomics: Iodoacetamide ikoreshwa mu ngamba zo gushyira ibimenyetso ku bipimo bya proteomics bishingiye kuri mass spectrometry. Binyuze mu buryo ikoresha hamwe n'ibisigazwa bya cysteine, ituma habaho gushyiraho no kumenya peptides zirimo cysteine.

Ubushakashatsi ku miterere ya poroteyine: Iodoacetamide ikoreshwa mu bushakashatsi ku miterere yayo kugira ngo ihindure ibisigazwa bya cysteine ​​mu buryo bw'imiti no kwiga imikorere yabyo n'uruhare rwabyo mu guhindura poroteyine, kuyihambira, no kuyihindura mu miterere yayo. Ishobora kandi kuba ingirakamaro mu bushakashatsi bwo guhuza poroteyine n'izindi.

Guhuza Peptides zifite Labels: Iodoacetamide ishobora gukoreshwa mu guhuza peptides zifite labels binyuze mu guhindura ibisigazwa bya cysteine ​​hamwe n'ibice bifite label ya isotopic, nka deuterium cyangwa karuboni-13. Izi peptides zifite labels zikoreshwa mu bushakashatsi butandukanye, harimo gupima poroteyine no kwiga dynamics.