Umukandara n'umuhanda: Ubufatanye, Ubwumvikane na Win-Win
ibicuruzwa

Ibicuruzwa

HEPES CAS: 7365-45-9 Igiciro cyabakora

HEPES (4- (2-Hydroxyethyl) piperazine-1-Ethanesulfonic aside) ni buffer biologiya ikoreshwa mubushakashatsi bwibinyabuzima na chimique.Azwiho ubushobozi bwo kugumana pH ihamye mubisubizo byamazi, bigatuma ibera mubikorwa bitandukanye birimo selile na enzymatique.HEPES ni uruganda rwa zwitterionic, bivuze ko rufite amafaranga meza kandi mabi kumatsinda atandukanye akora, abemerera kurwanya impinduka muri pH ziterwa no kongeramo acide cyangwa base.Ikoreshwa cyane mumico y'utugari, isesengura rya enzyme, ubushakashatsi bwa poroteyine, ubushakashatsi bwa electrophoreis, hamwe na farumasi.


Ibicuruzwa birambuye

Ibicuruzwa

Gushyira mu bikorwa n'ingaruka

pH buffering: HEPES ikoreshwa cyane nkibikoresho byo kubungabunga pH mu buryo butajegajega mu bitangazamakuru by’umuco w’akagari hamwe n’ibinyabuzima.Ikora neza murwego rwa physiologique pH ya 6.8 kugeza 8.2, bigatuma ikomeza kubungabunga ibihe byiza byubuzima.

Umuco w'akagari: HEPES ikunze kwongerwa mubitangazamakuru byumuco w'akagari kugirango ihagarike urwego rwa pH, rutanga ibidukikije bikwiye kugirango imikurire ikure kandi ikomeze kubaho neza.Ifasha gukumira ihindagurika rya pH rishobora kugira ingaruka mbi ku myitwarire ya selile nibisubizo byubushakashatsi.

Enzyme isobanura: HEPES ikunze gukoreshwa nka buffer mubitekerezo byimikorere bitewe nubushobozi bwayo bwo gukomeza pH yihariye.Ifasha mukubungabunga ibikorwa byimikorere myiza kandi itajegajega, bityo bikazamura ukuri nukuri kwizerwa rya enzyme.

Ubushakashatsi bwa poroteyine: HEPES ikoreshwa mubushakashatsi butandukanye bujyanye na poroteyine, harimo kweza poroteyine, korohereza poroteyine, no gusesengura imiterere ya poroteyine.Ifasha guhagarika pH no gukomeza poroteyine ikwiye kandi itajegajega muri ubu bushakashatsi.

Electrophoresis: HEPES isanga ikoreshwa mubuhanga bwa gel electrophorei, nka SDS-PAGE na agarose gel electrophorei.Ikoreshwa nkibikoresho byoherejwe muri buffer ikora, itanga ibidukikije bya pH bihamye byo gutandukana no gusesengura biomolecules.

Imiti ya farumasi: HEPES ikoreshwa mugutegura imiti yimiti n’ibinyabuzima.Ifasha kugumya gutuza no kuba inyangamugayo yibintu bikora, byemeza imikorere yabyo nubuzima bwiza.

Gupakira ibicuruzwa:

6892-68-8-3

Amakuru yinyongera:

Ibigize C8H18N2O4S
Suzuma 99%
Kugaragara Ifu yera
URUBANZA No. 7365-45-9
Gupakira Ntoya kandi nini
Ubuzima bwa Shelf Imyaka 2
Ububiko Ubike ahantu hakonje kandi humye
Icyemezo ISO.

 


  • Mbere:
  • Ibikurikira:

  • Andika ubutumwa bwawe hano hanyuma utwohereze