HEPES CAS: 7365-45-9

HEPES igira uruhare runini mu kubungabunga ibidukikije bya pH mu miterere y’uturemangingo, igenzura ko uturemangingo dushobora kubaho no gukura binyuze mu gukumira ihindagurika ry’urugero rwa pH. Ubu bushobozi bwo gufunga bwa HEPES ni ingenzi cyane mu kubungabunga imiterere y’umubiri mu bushakashatsi bushingiye ku turemangingo no gupima imiti, biganisha ku bisubizo byizewe kandi bishobora kongera kugaragara. Mu binyabuzima bya molekile na biochimie, HEPES ikoreshwa cyane mu gusuzuma enzymes kugira ngo igenzure neza uburyo enzymes zikora neza. Ubushobozi bwayo bwo kubungabunga pH idahinduka mu rugero ruto (ubusanzwe 7.2-7.6) ni ingenzi mu kwemeza ko inzira za biochemical zikora neza kandi neza, nko gutunganya poroteyine, gusesengura aside nucleic, no kwiga metabolic. HEPES ikoreshwa kandi mu gukoresha electrophoresis, harimo agarose gel electrophoresis na polyacrylamide gel electrophoresis, aho ifasha mu gushyiraho ibidukikije byiza byo gutandukanya ADN na poroteyine. Imiterere yayo yo gufunga igira uruhare mu migozi isobanutse neza kandi isobanutse neza, yongera ubushobozi n’ukuri kw’ibisubizo by’igerageza. Byongeye kandi, HEPES ikoreshwa cyane mu nganda z’imiti mu gukora ibikomoka ku binyabuzima, inkingo, n’ibikoreshwa mu gusuzuma. Kuba ihura n’ibinyabuzima bifite ubushobozi bwo gukora ibintu bitandukanye ndetse n’uburozi buke mu mubiri bituma iba amahitamo meza yo kubungabunga umutekano n’imikorere y’imiti mu gihe cyo kuyitunganya no kuyibika. Muri rusange, HEPES ikora nk'ikintu cy’ingenzi kandi gifasha mu kugabanya ubukana bw’imiti, ikaba ifite akamaro gatandukanye mu bushakashatsi ku ngirabuzimafatizo, ubushakashatsi ku ngirabuzimafatizo, ubuvuzi bwa siyansi, no guteza imbere imiti, ikaba igira uruhare runini mu kwemeza ko igerageza ry’ibintu bitandukanye mu bijyanye n’ibinyabuzima n’imiti rigenda neza kandi ryizewe.