Umunyu wa Gly-Pro p-nitroanilide p-toluenesulfonate CAS: 65096-46-0

Ingaruka: Umunyu wa Gly-Pro p-nitroanilide p-toluenesulfonate wagenewe by'umwihariko kuba substrate ya peptidases yihariye ya proline. Izi enzymes zitandukanya umugozi wa peptide hagati ya glycine (Gly) na proline (Pro), zigasohora p-nitroanilide (pNA) nk'umusaruro. Molekile ya pNA ifata urumuri ku bunini bw'uburebure runaka, bigatuma hapimwa pNA yarekuwe kandi hagapimwa imikorere ya enzyme.

Imikoreshereze: Umunyu wa Gly-Pro p-nitroanilide p-toluenesulfonate ukoreshwa mu nzego zitandukanye z'ubushakashatsi, harimo biochimie, enzymology, no kuvumbura imiti, mu bikorwa bikurikira:

Isuzuma ry'imikorere ya enzyme: Rikoreshwa cyane mu gupima imikorere ya peptidases yihariye ya proline, harimo dipeptidyl peptidases (DPPs) na prolyl endopeptidases (PEPs).

Imiti igabanya imisemburo: Umunyu wa Gly-Pro p-nitroanilide p-toluenesulfonate ushobora gukoreshwa mu gusuzuma ubushobozi bw'ibinyabutabire cyangwa imiti ku musaruro wa peptidase wihariye wa proline. Kugabanuka k'irekurwa rya pNA bigaragaza ko hari umuti ugabanya imisemburo.

Inyigo ku bijyanye na enzyme: Mu guhindura ingano y'umunyu wa Gly-Pro p-nitroanilide p-toluenesulfonate no gupima umuvuduko w'irekurwa rya pNA, ibipimo bya enzyme kinetics nka Km (Michaelis-Menten constant) na Vmax (umuvuduko ntarengwa) bishobora kumenyekana.

Uburyo bwo gusuzuma: Imikorere ya peptidase zihariye za proline ishobora kugira akamaro mu gusuzuma indwara n'ibindi bibazo bitandukanye, nk'indwara zandurira mu maraso na kanseri. Umunyu wa Gly-Pro p-nitroanilide p-toluenesulfonate ushobora gukoreshwa nk'igikoresho cyo gusuzuma urwego rw'imikorere y'izi enzymes mu barwayi.